Ribeiro Michelle M A C, Oliveira Thiago C, Batista Alex D, Muñoz Rodrigo A A, Richter Eduardo M
Instituto de Química, Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, 13400-970, Uberlândia, MG, Brazil.
Instituto de Química, Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121, 13400-970, Uberlândia, MG, Brazil.
J Chromatogr A. 2016 Nov 11;1472:134-137. doi: 10.1016/j.chroma.2016.10.031. Epub 2016 Oct 12.
This paper reports for the first time, a method for simultaneous determination of naphazoline (NPZ) and zinc (Zn) using an analytical separation technique (capillary electrophoresis with capacitively coupled contactless conductivity detection -CE-CD). A single run is possible every 55s (sampling rate=65h). The separation by CE-CD was achieved on a fused silica capillary (50cm length - 10cm effective, 50μm i.d.) with a background electrolyte (BGE) composed by 20mmolL of 2-(morpholin-4-yl)ethane-1-sulfonic acid (MES) and 20mmolL of histidine (HIS) (pH 6.0). Detection limits were estimated at 20 and 30μmolL and recovery values for spiked samples were 98 and 102% for NPZ and Zn, respectively. The developed procedure was compared to HPLC (NPZ) and FAAS (Zn) and no statistically significant differences were observed (95% confidence level).
本文首次报道了一种使用分析分离技术（电容耦合非接触电导检测毛细管电泳-CE-CD）同时测定萘甲唑啉（NPZ）和锌（Zn）的方法。每55秒可进行一次单次运行（采样率=65小时）。通过CE-CD在熔融石英毛细管（长度50cm-有效长度10cm，内径50μm）上实现分离，背景电解质（BGE）由20mmol/L的2-（吗啉-4-基）乙烷-1-磺酸（MES）和20mmol/L的组氨酸（HIS）（pH 6.0）组成。检测限估计为20和30μmol/L，加标样品中NPZ和Zn的回收率分别为98%和102%。将所开发的方法与HPLC（用于NPZ）和FAAS（用于Zn）进行比较，未观察到统计学上的显著差异（95%置信水平）。
