Sakamoto W, Yoshikawa K, Shindoh M, Amemiya A, Handa H, Saeki T, Nagasawa S, Koyama J, Ogihara T, Mino M
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Int J Vitam Nutr Res. 1989;59(2):131-9.
In order to clarify the modulating effect on macrophages by vitamin E, we investigated the in vivo effects of vitamin E on the peritoneal macrophages and T-kininogen level in rats. Peritoneal injections of vitamin E for 6 successive days resulted in a significant increase of leucocytes, mostly consisting of macrophages, in the peritoneal cavity. Namely, in the case of 5 mg vitamin E per rat (400 g body weight), the number of leucocytes in the peritoneal cavity and T-kininogen level in plasma showed 171.57 +/- 15.96 X 10(6) per rat and 1372 +/- 364 micrograms/ml, whereas the placebo vehicle was 28.54 +/- 8.24 X 10(6) and 315 +/- 130, respectively. Macrophages, in which alpha-tocopherol contained approximately 158.2 ng/10(6) cells after exogenous vitamin E treatment, showed less acid phosphatase activity and less O2- generation than that treated with placebo vehicle. In contrast, the acid phosphatase activity of cellfree peritoneal exudate fluid with vitamin E increased approximately two to three-fold as compared to the placebo vehicle. From these results, we discussed the relationship between in vivo effects of vitamin E on peritoneal macrophages and T-kininogen induction.
为了阐明维生素E对巨噬细胞的调节作用，我们研究了维生素E对大鼠腹腔巨噬细胞和T -激肽原水平的体内影响。连续6天腹腔注射维生素E导致腹腔内白细胞显著增加，其中大多数为巨噬细胞。也就是说，对于每只体重400克的大鼠注射5毫克维生素E的情况，腹腔内白细胞数量和血浆中T -激肽原水平分别为每只大鼠171.57±15.96×10⁶个和1372±364微克/毫升，而安慰剂组分别为28.54±8.24×10⁶个和315±130。在外源性维生素E处理后，α-生育酚含量约为158.2纳克/10⁶个细胞的巨噬细胞，其酸性磷酸酶活性和O₂⁻生成量比用安慰剂处理的巨噬细胞少。相反，与安慰剂组相比，含维生素E的无细胞腹腔渗出液的酸性磷酸酶活性增加了约两到三倍。根据这些结果，我们讨论了维生素E对腹腔巨噬细胞的体内作用与T -激肽原诱导之间的关系。
