Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and College of Engineering, Peking University, Beijing, China.
School of Electronics Engineering and Computer Science, Peking University, Beijing, China.
Lab Chip. 2016 Nov 15;16(23):4512-4516. doi: 10.1039/c6lc01084a.
Many on-chip approaches that use flow-focusing to pinch the continuous aqueous phase into droplets have become the most popular methods that provide monodisperse emulsion droplets. However, not every lab can easily adapt a microfluidic workflow into their familiar protocols. We develop an off-chip approach, spinning micro-pipette liquid emulsion (SiMPLE) generator, to produce highly stable monodisperse water-in-oil emulsions using a moving micropipette to disperse the aqueous phase in an oil-filled microcentrifuge tube. This method provides a simple way to produce picoliter-size droplets in situ with no dead volume during emulsification. With SiMPLE, single-cell emulsion whole genome amplification was performed to demonstrate that this novel method can seamlessly be integrated with experimental operations and supplies that most researchers are familiar with. The SiMPLE generator has effectively lowered the technical difficulties in applications relying on emulsion droplets.
许多使用流聚焦将连续水相挤压成液滴的片上方法已成为提供单分散乳液液滴的最受欢迎的方法。然而，并非每个实验室都能轻松地将微流控工作流程应用于其熟悉的方案中。我们开发了一种片外方法，旋转微管液体乳液（SiMPLE）发生器，使用移动微管在充满油的微离心管中分散水相，以产生高度稳定的单分散油包水乳状液。该方法提供了一种简单的方法，可以在乳化过程中无死体积就地产生皮升级别大小的液滴。使用 SiMPLE 进行单细胞乳液全基因组扩增，证明这种新方法可以与大多数研究人员熟悉的实验操作和供应无缝集成。SiMPLE 发生器有效地降低了依赖乳液液滴的应用中的技术难度。
