Korniluk Aleksandra, Kamińska Joanna, Kiszło Paweł, Kemona Halina, Dymicka-Piekarska Violetta
a Department of Clinical Laboratory Diagnostics , Medical University of Bialystok , Poland.
Biomarkers. 2017 Nov;22(7):629-634. doi: 10.1080/1354750X.2016.1252967. Epub 2016 Nov 9.
Selectins probably participate in the interactions between platelets and other inflammatory cells in cancer invasion and metastasis formation. We assessed a potential relationship of P-, L- and E-selectin in colorectal cancer (CRC) patients in relation to tumour advancement according to TNM classification, and tumour location.
The study group was composed of 53 CRC patients and 25 healthy subjects. Plasma levels of soluble P-, L- and E-selectins were measured using the immunoenzymatic method with Quantikine kits (R&D Systems, Minneapolis, MN).
The mean levels of all selectins were significantly higher in CRC patients compared to healthy controls. The highest level of sP-selectin was observed in patients with metastases to the liver (stage IV), and was significantly higher than in patients without metastases (stage I/II) and with lymph node metastases (stage III), p = .02. The highest levels of sL- and sE-selectin were observed in patients with lymph node metastasis. We also found sP-selectin to be the best predictor of CRC.
Our finding show possible involvement of tested selectins in CRC advancement and forming metastasis. Among sL- and E- selectins, P-selectin plays an important role in the progression of CRC and could be an attractive biomarker with clinical significance.
在癌症侵袭和转移形成过程中，选择素可能参与血小板与其他炎症细胞之间的相互作用。我们根据TNM分类和肿瘤位置，评估了P-、L-和E-选择素在结直肠癌（CRC）患者中与肿瘤进展的潜在关系。
研究组由53例CRC患者和25名健康受试者组成。使用Quantikine试剂盒（R&D Systems，明尼阿波利斯，明尼苏达州）通过免疫酶法测量血浆中可溶性P-、L-和E-选择素的水平。
与健康对照组相比，CRC患者中所有选择素的平均水平均显著更高。在发生肝转移（IV期）的患者中观察到sP-选择素水平最高，且显著高于无转移（I/II期）和有淋巴结转移（III期）的患者，p = 0.02。在有淋巴结转移的患者中观察到sL-和sE-选择素水平最高。我们还发现sP-选择素是CRC的最佳预测指标。
我们的研究结果表明，所检测的选择素可能参与了CRC的进展和转移形成。在sL-和E-选择素中，P-选择素在CRC进展中起重要作用，可能是一种具有临床意义的有吸引力的生物标志物。
