Qi Meng, Wang Rongjun, Wang Haiyan, Jian Fuchun, Li Junqiang, Zhao Jinfeng, Dong Haiju, Zhu Huili, Ning Changshen, Zhang Longxian
College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95 Wenhua Road, Zhengzhou, 450002, China.
College of Animal Science, Tarim University, 1487 Tarim Road, Alar, 843300, China.
J Eukaryot Microbiol. 2016 Sep;63(5):591-7. doi: 10.1111/jeu.12308. Epub 2016 Mar 8.
In present study, 262 fecal specimens were collected from 12 groups of grazing horses in the Xinjiang Uyghur Autonomous Region of China. The specimens were subjected to PCR and sequencing analyses of the ribosomal internal transcribed spacer (ITS). The overall prevalence of E. bieneusi in horses was 30.9% (81/262). No significant differences in prevalence were observed between horses of different ages or sexes. Nineteen genotypes were identified: 15 known genotypes (BEB6, CHG19, CM6, CM7, CM8, CS-1, CS-4, D, EpbA, EbpC, G, horse1, horse2, O, and Peru8) and four new genotypes (XJH1-XJH4). Six of these genotypes were previously detected in humans: BEB6, D, EbpA, EbpC, O, and Peru8. Genotype EbpC was the most prevalent (21/81), followed by EpbA (20/81), BEB6 (9/81), CM6 (4/81), horse1 (4/81), O (4/81), G (3/81), CHG19 (2/81), CM7 (2/81), horse2 (2/81), and XJH1 (2/81), whereas the remaining eight genotypes were seen in one specimen each. In a phylogenetic analysis, 14 genotypes (65/81, 80.2%), excluding genotypes BEB6, CM7, horse2, XJH1, and XJH4, belonged to group 1, which have zoonotic potential. The high diversity in the E. bieneusi genotypes and their zoonotic potential suggest that grazing horses are a potential source of zoonotic infection in humans.
在本研究中，从中国新疆维吾尔自治区12组放牧马匹中采集了262份粪便样本。对样本进行核糖体内部转录间隔区（ITS）的PCR和测序分析。马匹中微小隐孢子虫的总体感染率为30.9%（81/262）。不同年龄或性别的马匹在感染率上未观察到显著差异。鉴定出19种基因型：15种已知基因型（BEB6、CHG19、CM6、CM7、CM8、CS-1、CS-4、D、EpbA、EbpC、G、horse1、horse2、O和Peru8）和4种新基因型（XJH1-XJH4）。其中6种基因型先前在人类中被检测到：BEB6、D、EpbA、EbpC、O和Peru8。基因型EbpC最为常见（21/81），其次是EpbA（20/81）、BEB6（9/81）、CM6（4/81）、horse1（4/81）、O（4/81）、G（3/81）、CHG19（2/81）、CM7（2/81）、horse2（2/81）和XJH1（2/81），而其余8种基因型各在1个样本中出现。在系统发育分析中，排除基因型BEB6、CM7、horse2、XJH1和XJH4后，14种基因型（65/81，80.2%）属于第1组，具有人畜共患病潜力。微小隐孢子虫基因型的高度多样性及其人畜共患病潜力表明，放牧马匹是人类人畜共患感染的潜在来源。
