Ji Li-Li, Guo Meng-Wei, Ren Xiu-Jun, Ge Dong-Yu, Li Gen-Mao, Tu Ya
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 100029, China.
School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing, 100029, China.
Chin J Integr Med. 2017 Oct;23(10):786-792. doi: 10.1007/s11655-016-2606-y. Epub 2016 Oct 24.
To investigate the effect of electroacupuncture (EA) treatment on the expression of cyclooxygenase (COX) 2 and microglia in spinal cord by using rat model of neuropathic pain, and to probe into the relationship between COX 2 and microglia.
The rats were randomly divided into 6 groups, including normal control group, model group, sham group, EA 1 group (distant acupoints + local acupoints), EA 2 group (local acupoints), and EA 3 group (distant acupoints). Thermal withdrawal latencies were evaluated at 1 day preoperatively and 3, 5 and 7 days postoperatively. At 7 days postoperatively, the spinal COX 2 mRNA was detected by reverse-transcription polymerase chain reaction. Double immunofluorescent staining technology was applied to screen and verify the relationship between altered COX 2 and microglia.
Compared with the model group, thermal withdrawal latencies increased after EA treatment (P<0.01). The expressions of COX 2 mRNA were up-regulated in spinal cord of rat on day 7 after surgery (P<0.05). Compared with the model group, EA stimulation (EA 1 and EA 2 groups) reversed the up-regulation of COX 2 mRNA expression (P<0.05). EA 1 and EA 2 groups might have better treatment effect compared with the EA 3 group. Fluorescent images displayed COX 2 and microglia expressed at common areas.
EA was effective in analgesic and anti-inflammatory. EA has decreased the expression of spinal COX 2 mRNA in the trend of the therapeutic effect of "distant acupoints + local acupoints", and "local acupoints" intervention may be superior to that of "distant acupoints" intervention. Microglia may be related to the formation of COX 2.
采用神经性疼痛大鼠模型，探讨电针（EA）治疗对脊髓中环氧合酶（COX）-2表达及小胶质细胞的影响，并探究COX-2与小胶质细胞之间的关系。
将大鼠随机分为6组，包括正常对照组、模型组、假手术组、EA 1组（远部穴位+局部穴位）、EA 2组（局部穴位）和EA 3组（远部穴位）。于术前1天及术后3、5和7天评估热缩足潜伏期。术后7天，采用逆转录聚合酶链反应检测脊髓COX-2 mRNA。应用双重免疫荧光染色技术筛选并验证COX-2改变与小胶质细胞之间的关系。
与模型组相比，电针治疗后热缩足潜伏期延长（P<0.01）。术后第7天，大鼠脊髓中COX-2 mRNA表达上调（P<0.05）。与模型组相比，电针刺激（EA 1组和EA 2组）可逆转COX-2 mRNA表达的上调（P<0.05）。与EA 3组相比，EA 1组和EA 2组可能具有更好的治疗效果。荧光图像显示COX-2与小胶质细胞在共同区域表达。
电针具有镇痛和抗炎作用。电针在“远部穴位+局部穴位”及“局部穴位”干预的治疗作用趋势下降低了脊髓COX-2 mRNA的表达，且“局部穴位”干预可能优于“远部穴位”干预。小胶质细胞可能与COX-2的形成有关。
