化学蛋白质组学应用中的可裂解连接子
Cleavable Linkers in Chemical Proteomics Applications.
作者信息
Yang Yinliang, Fonović Marko, Verhelst Steven H L
机构信息
Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany.
Department of Biochemistry, Molecular and Structural Biology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana, Slovenia.
出版信息
Methods Mol Biol. 2017;1491:185-203. doi: 10.1007/978-1-4939-6439-0_14.
The discovery of the protein targets of small molecule probes is a crucial aspect of activity-based protein profiling and chemical biology. Mass spectrometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their different chemistries, and describe how they aid in protein identification.
小分子探针的蛋白质靶点发现是基于活性的蛋白质谱分析和化学生物学的一个关键方面。质谱分析法是靶点识别的主要方法，在过去十年中，可裂解连接子已成为促进蛋白质富集和识别的常用策略。在本章中，我们概述了化学蛋白质组学方法中使用的可裂解连接子，讨论了它们不同的化学性质，并描述了它们如何辅助蛋白质识别。
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