BMP-2、BMP-4及其受体BMPR-IA和BMPR-II在成釉细胞瘤和腺样牙源性肿瘤中的免疫表达。

Immunoexpression of BMP-2 and BMP-4 and their receptors, BMPR-IA and BMPR-II, in ameloblastomas and adenomatoid odontogenic tumors.

作者信息

Nascimento Marcelo Anderson Barbosa, Nonaka Cassiano Francisco Weege, Barboza Carlos Augusto Galvão, Freitas Roseana de Almeida, Pereira Pinto Leão, Souza Lélia Batista de

机构信息

Postgraduate Program in Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

Department of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil.

出版信息

Arch Oral Biol. 2017 Jan;73:223-229. doi: 10.1016/j.archoralbio.2016.10.022. Epub 2016 Oct 19.

DOI:10.1016/j.archoralbio.2016.10.022

PMID:27780042

Abstract

OBJECTIVES

The present study evaluated the immunohistochemical expression of BMP-2 and BMP-4 and of their receptors (BMPR-IA and BMPR-II) in solid ameloblastoma (SA), unicystic ameloblastoma (UA) and adenomatoid odontogenic tumor (AOT) in order to obtain a better understanding of their role in the development and biological behavior of these tumors.

DESIGN

This study analyzed these proteins in 30 cases of SA, 10 cases of UA, and 30 cases of AOT. Immunoexpression was evaluated in the parenchyma and stroma by attributing the following scores: 0, no stained cells; 1, ≤10%; 2, >10% and ≤25%; 3, >25% and ≤50%; 4, >50% and ≤75%.; 5, >75% stained cells.

RESULTS

In SAs, positive correlations were observed between the stromal and parenchymal expression of BMP-2 (p<0.001) and between the stromal expression of BMP-2 and BMP-4 (p=0.020), as well as between the stromal expression of BMPR-II and BMP-4 (p=0.001) and the stromal and parenchymal expression of BMPR-II (p<0.001). In UAs, correlations were detected between the stromal and parenchymal expression of BMP-4 (p=0.035) and between the stromal expression of BMP-4 and BMPR-IA (p=0.022). In AOTs, analysis of immunoexpression in the parenchyma revealed positive correlations between all proteins.

CONCLUSION

BMPs and their receptors play an important role in the differentiation and development of ameloblastomas and AOTs, but may not explain the different biological behaviors of these lesions. The positive correlation observed in AOTs might be related to the formation of mineralized material in this tumor.

摘要

目的

本研究评估了骨形态发生蛋白-2（BMP-2）和骨形态发生蛋白-4（BMP-4）及其受体（BMPR-IA和BMPR-II）在实性成釉细胞瘤（SA）、单囊性成釉细胞瘤（UA）和腺样牙源性肿瘤（AOT）中的免疫组化表达情况，以便更好地了解它们在这些肿瘤发生发展及生物学行为中的作用。

设计

本研究分析了30例SA、10例UA和30例AOT中的这些蛋白。通过赋予以下评分来评估实质和间质中的免疫表达：0，无染色细胞；1，≤10%；2，>10%且≤25%；3，>25%且≤50%；4，>50%且≤75%；5，>75%染色细胞。

结果

在SA中，观察到BMP-2的间质与实质表达之间存在正相关（p<0.001），BMP-2与BMP-4的间质表达之间存在正相关（p=0.020），以及BMPR-II与BMP-4的间质表达之间存在正相关（p=0.001），BMPR-II的间质与实质表达之间存在正相关（p<0.001）。在UA中，检测到BMP-4的间质与实质表达之间存在正相关（p=0.035），BMP-4与BMPR-IA的间质表达之间存在正相关（p=0.022）。在AOT中，对实质免疫表达的分析显示所有蛋白之间均存在正相关。