Jimenez D A, Chandler J E, Adkinson R W, Nipper W A, Baham A, Saxton A M
Louisiana State University Agricultural Center, Baton Rouge 70803.
J Dairy Sci. 1989 Jul;72(7):1866-75. doi: 10.3168/jds.S0022-0302(89)79305-X.
Yearling Holstein bulls were fed a corn silage ration supplemented with either cottonseed meal with gossypol or soybean meal in two trials to evaluate the effect of feeding gossypol on reproductive characteristics. In Trial 1, roughage to concentrate ratio was 88:12 and was fed for 60 d. In Trial 2, roughage to concentrate ratio was 50:50 and was fed for 42 d. Cottonseed meal concentrate had 3.03 g total gossypol/kg DM. Cottonseed meal concentrate was fed to provide 6 and 30 mg total gossypol/kg BW per d in Trials 1 and 2. Ejaculates were collected twice weekly via artificial vagina and critiqued for quantity and quality before and after thawing and after postthaw incubation. Leptotene spermatocytes to Sertoli cell ratio in stage 1 tubules was used to evaluate spermatogenesis. Growth characteristics and tissue total gossypol concentrations were also evaluated. No gossypol was found in plasma taken before, during, or after Trial 1 or from body organs or plasma taken during or after Trial 2. No signs of gossypol toxicity were observed, and growth characteristics were similar on both rations. Gossypol in cottonseed meal fed at low to moderate concentrations was not deleterious to seminal quantity or quality, and spermatogenesis was unaffected by treatment.
在两项试验中，给一岁的荷斯坦公牛饲喂添加了含游离棉酚的棉籽粕或豆粕的玉米青贮日粮，以评估饲喂棉酚对繁殖特性的影响。在试验1中，粗饲料与精饲料的比例为88:12，饲喂60天。在试验2中，粗饲料与精饲料的比例为50:50，饲喂42天。棉籽粕浓缩物中总棉酚含量为3.03克/千克干物质。在试验1和试验2中，饲喂棉籽粕浓缩物以提供每天每千克体重6毫克和30毫克的总棉酚。每周通过人工阴道采集两次精液，并在解冻前后和解冻后孵育后对精液的数量和质量进行评估。使用1期曲细精管中的细线期精母细胞与支持细胞的比例来评估精子发生。还评估了生长特性和组织总棉酚浓度。在试验1之前、期间或之后采集的血浆中，以及在试验2期间或之后采集的身体器官或血浆中均未发现棉酚。未观察到棉酚中毒的迹象，两种日粮的生长特性相似。低至中等浓度饲喂的棉籽粕中的棉酚对精液数量或质量无害，精子发生不受处理的影响。
