治疗性基因FCU1的表达使胰腺癌细胞对5-氟胞嘧啶敏感并增强5-氟尿嘧啶的细胞毒性作用。
Expression of Therapeutic Gene FCU1 Sensitizes Pancreatic Cancer Cells to 5-Fluorocytosine and Enhances the Cytotoxic Effect of 5-Fluorouracil.
作者信息
Kopantsev E P, Kostina M B, Grankina E V, Kopantseva M R, Egorov V I, Sverdlov E D
机构信息
Laboratory of Structure and Functions of Human Genes, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
A. V. Vishnevsky Institute of Surgery, Ministry of the Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2016 Oct;161(6):808-810. doi: 10.1007/s10517-016-3517-9. Epub 2016 Oct 26.
Hybrid therapeutic gene FCU1 gene was cloned into a lentiviral expression vector and the therapeutic effect of its expression was studied in three pancreatic cancer cell lines. Expression of FCU1 gene sensitized cells of two of three studied pancreatic cancer cell lines to 5-fluorocytosine. In addition, uracil phosphoribosyl transferase activity of the hybrid FCU1 protein increased sensitivity of transfected cells of all three studied pancreatic cancer cell lines to 5-fluorouracil, a standard chemotherapeutic agent.
将杂交治疗基因FCU1基因克隆到慢病毒表达载体中，并在三种胰腺癌细胞系中研究其表达的治疗效果。FCU1基因的表达使三种研究的胰腺癌细胞系中的两种细胞系对5-氟胞嘧啶敏感。此外，杂交FCU1蛋白的尿嘧啶磷酸核糖转移酶活性提高了所有三种研究的胰腺癌细胞系的转染细胞对标准化疗药物5-氟尿嘧啶的敏感性。
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