Salifu A A, Lekakou C, Labeed F
Advanced Materials Centre, University of Surrey, Guildford, GU2 7XH, United Kingdom.
Biomedical Engineering Centre, University of Surrey, Guildford, GU2 7XH, United Kingdom.
J Biomed Mater Res A. 2017 Mar;105(3):779-789. doi: 10.1002/jbm.a.35954. Epub 2016 Nov 18.
Multilayer cellular stacks of crosslinked, electrospun 25 wt % hydroxyapatite (HA)-gelatin and pure gelatin fiber scaffolds, seeded with human fetal osteoblasts (hFOBs), were studied for up to 18 days in static and dynamic cell culture. Two types of stack models were investigated: a four-layer stack with cells seeded at the bottom surface of the first/top layer and the top surface of the fourth/bottom layer, so that the two middle layers were not seeded with cells with the aim to act as continuing conduits of culture medium and nutrients supply to the adjacent cell-populated zones; a three-layer stack with cells seeded at the bottom surface of each layer. hFOBs exhibited lower migration rate through the stack thickness for the 25 wt % HA-gelatin scaffolds as compared to the pure gelatin scaffolds, due to the small pores of the former. Hence, the regularly seeded three-layer stack maintained cell-free porous zones in all layers through which the culture medium could continuously perfuse, while good fusion was achieved at the interface of all layers via the cross-migrating cells with a preference to downwards vertical migration attributed to gravity. Dynamic cell culture conditions enhanced overall cell growth by about 6% for the regularly seeded three-layer stack. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 779-789, 2017.
研究人员将人胎儿成骨细胞（hFOB）接种到交联电纺25 wt%羟基磷灰石（HA）-明胶和纯明胶纤维支架的多层细胞堆叠体上，在静态和动态细胞培养中培养长达18天。研究了两种堆叠模型：一种是四层堆叠体，细胞接种在第一层/顶层的底面和第四层/底层的顶面，这样中间两层不接种细胞，目的是作为培养基和营养物质供应到相邻细胞填充区域的连续通道；另一种是三层堆叠体，每层底面都接种细胞。与纯明胶支架相比，由于25 wt% HA-明胶支架的孔隙较小，hFOB在堆叠体厚度方向上的迁移速率较低。因此，规则接种的三层堆叠体在所有层中都保持无细胞的多孔区域，培养基可以通过这些区域持续灌注，同时通过交叉迁移的细胞在所有层的界面处实现良好融合，这些细胞由于重力作用更倾向于向下垂直迁移。动态细胞培养条件使规则接种的三层堆叠体的总体细胞生长提高了约6%。© 2016威利期刊公司。《生物医学材料研究杂志》A部分：105A：779 - 789，2017年。
