Shi Rui, Guo Su-Qing, Liu Shan, Li Zhi-Shang, Li Jun-Jun
Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China.
Department of Hematology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Oct;24(5):1339-1342. doi: 10.7534/j.issn.1009-2137.2016.05.010.
To investigate the anti-angiogenesis effect of Scutellaria barbata extract(SBE) on chronic myeloid leukemia(CML) K562 cell line in vitro.
The proliferating activity after treating K562 cells with 0.5,1.0,2.0 and 4.0 g/ml SB for 24, 36, 48 hours were assessed by MTT assay. The level of vascular endothelial growth factor(VEGF) in the culture supematant of K562 cells was determined by ELISA; and the expression of VEGF mRNA was detected by RT-PCR.
MTT assay showed that SBE could inhibit the proliferation of K562 cells in a dose-dependent manner (r=0.56); ELISA displayed that the concentration of VEGF in K562 cells in blank-control group was most high; after intervention of K562 cells by SBE (0.5,1.0,2.0 and 4.0 g/ml) for 48 h, the concentration of VEGF decreased, the comparison between different groups showed significant differences (P<0.05); after treatment with SBE for 48 h, the expression of VEGF mRNA in K562 cells decreased, the gray scale ratio of target gene/β-actin declined, and the difference between various groups was statistically significant (P<0.05). Conclution: SBE can inhibit K562 cell proliferation, its action mechanism may related with the VEGF level concentration in K562 cells and down-regulation of VEGF mRNA expression.
