Kubota M, Ito Y, Taguchi T, Ikeda K, Ikadai H
Department of Pediatric Surgery, Kyusyu University, Fukuoka, Japan.
J Pediatr Surg. 1989 Sep;24(9):911-9. doi: 10.1016/s0022-3468(89)80594-9.
The pattern of innervation in the aganglionic colon and internal anal sphincter from cogenitally aganglionic rats was studied and compared with that of control littermates. In normoganglionic colon and anal sphincter, electrical stimulation evoked excitatory or inhibitory junction potentials followed by a contraction or relaxation, respectively. These responses were abolished by tetrodotoxin and atropine selectively abolished the excitatory effects, indicating that the colon or anal sphincter is innervated by intrinsic cholinergic excitatory and noncholinergic inhibitory nerves. In congenitally aganglionic rats, electrical stimulation evoked excitatory and inhibitory responses in sphincteric regions, while only excitatory responses were observed in distal segments. Excitatory responses were weak in proximal segments of the aganglionic colon and electrical stimulation failed to evoke neurogenic responses. These results indicate regional differences in the functional innervation of extrinsic nerve fibers in the aganglionic colon from congenitally aganglionic rats and the usefulness of congenitally aganglionic rats as an animal model for Hirschsprung's disease.
研究了先天性无神经节大鼠无神经节结肠和肛门内括约肌的神经支配模式，并与对照同窝仔鼠进行了比较。在正常神经节的结肠和肛门括约肌中，电刺激分别诱发兴奋性或抑制性接头电位，随后分别引起收缩或舒张。这些反应被河豚毒素消除，阿托品选择性地消除了兴奋性效应，表明结肠或肛门括约肌由内在胆碱能兴奋性神经和非胆碱能抑制性神经支配。在先天性无神经节大鼠中，电刺激在括约肌区域诱发兴奋性和抑制性反应，而在远端节段仅观察到兴奋性反应。无神经节结肠近端节段的兴奋性反应较弱，电刺激未能诱发神经源性反应。这些结果表明先天性无神经节大鼠无神经节结肠中外周神经纤维功能神经支配的区域差异，以及先天性无神经节大鼠作为先天性巨结肠动物模型的实用性。
