Nakagawa H, Sato K, Miyai H, Yamamoto Y
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
J Pharmacobiodyn. 1989 Jun;12(6):363-9. doi: 10.1248/bpb1978.12.363.
Rat serum obtained at 24 h after subcutaneous injection of carrageenin significantly suppressed chemotaxis of rat polymorphonuclear leukocytes (PMNs) in vitro. alpha 2 Acute-phase macroglobulin (alpha 2APM), alpha 1 proteinase inhibitor (alpha 1 PI) and cysteine-proteinase inhibitors (CPIs) are present at high concentration in the 24-h serum and known as acute-phase reactants in rats. These acute-phase proteinase inhibitors were purified from inflamed rat serum or exudate and their effect on PMN chemotaxis was studied by Boyden's method in vitro. alpha 2 APM (4 mg/ml) significantly suppressed PMN chemotaxis while alpha 1M was without effect, though both alpha 2APM and alpha 1M had a similar anti-proteinase activity. The results suggest that alpha 2APM suppressed PMN chemotaxis through the mechanism unrelated to its anti-proteinase activity. On the other hand, alpha 1PI (1 and 3 mg/ml) slightly but significantly suppressed PMN chemotaxis, whereas CPI-1 and CPI-2 had no inhibitory effect. These results suggest that alpha 2APM and alpha 1PI play a role in suppression of PMNs infiltration into the inflammatory site in the late-phase of acute inflammation.
皮下注射角叉菜胶24小时后获得的大鼠血清在体外显著抑制大鼠多形核白细胞（PMN）的趋化性。α2急性期巨球蛋白（α2APM）、α1蛋白酶抑制剂（α1PI）和半胱氨酸蛋白酶抑制剂（CPI）在24小时血清中浓度很高，在大鼠中被称为急性期反应物。这些急性期蛋白酶抑制剂从炎症大鼠血清或渗出物中纯化出来，并用博伊登体外法研究它们对PMN趋化性的影响。α2APM（4毫克/毫升）显著抑制PMN趋化性，而α1M无作用，尽管α2APM和α1M具有相似的抗蛋白酶活性。结果表明，α2APM通过与其抗蛋白酶活性无关的机制抑制PMN趋化性。另一方面，α1PI（1和3毫克/毫升）轻微但显著抑制PMN趋化性，而CPI-1和CPI-2没有抑制作用。这些结果表明，α2APM和α1PI在急性炎症后期抑制PMN浸润到炎症部位中发挥作用。
