Khan Samiya, Adhikari D K, Gupta Sanjay, Gupta Nidhi
Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., 201301, India.
Biofuels Division & HOA Biotechnology Conversion Area, Indian Institute of Petroleum, Mohkampur, Dehradun, 248005, India.
Biotechnol Lett. 2017 Feb;39(2):277-281. doi: 10.1007/s10529-016-2242-9. Epub 2016 Oct 27.
With the view of designing a single biocatalyst for biorefining, carbazole dioxygenase was cloned from Pseudomonas sp. and expressed in Rhodococcus sp.
The recombinant, IGTS8, degraded both carbazole and dibenzothiophene at 400 mg/l in 24 h. Maximum carbazole degradation was in 1:1 (v/v) hexadecane/aqueous phase. Anthracene, phenanthrene, pyrene, fluoranthene and fluorine were also degraded without affecting the aliphatic component.
Recombinant Rhodococcus sp. IGTS8 can function as a single biocatalyst for removing major contaminants of fossil fuels viz. dibenzothiophene, carbazole and polyaromatic compounds.
为了设计一种用于生物精炼的单一生物催化剂，从假单胞菌属中克隆咔唑双加氧酶并在红球菌属中表达。
重组体IGTS8在24小时内将咔唑和二苯并噻吩的浓度均降解至400mg/l。咔唑的最大降解量出现在十六烷/水相体积比为1:1（v/v）的体系中。蒽、菲、芘、荧蒽和氟也能被降解，且不影响脂肪族成分。
重组红球菌属IGTS8可作为一种单一生物催化剂，用于去除化石燃料中的主要污染物，即二苯并噻吩、咔唑和多环芳烃化合物。
Zotero 插件