Thompson Dean, Cognat Valérie, Goodfellow Michael, Koechler Sandrine, Heintz Dimitri, Carapito Christine, Van Dorsselaer Alain, Mahmoud Huda, Sangal Vartul, Ismail Wael
Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom.
Institut de Biologie Moléculaire des Plantes, Centre National de Recherche Scientifique (CNRS), Université de Strasbourg, Strasbourg, France.
Front Microbiol. 2020 Jul 7;11:1417. doi: 10.3389/fmicb.2020.01417. eCollection 2020.
strain IGTS8 is the most extensively studied model bacterium for biodesulfurization of fossil fuels via the non-destructive sulfur-specific 4S pathway. This strain was initially assigned to and later to thus making its taxonomic status debatable and reflecting the limited resolution of methods available at the time. In this study, phylogenomic analyses of the whole genome sequences of strain IGTS8 and closely related rhodococci showed that and are very closely related species, that strain IGTS8 is a strain and that several strains identified as should be re-classified as . The genomes of strains assigned to these species contain potentially novel biosynthetic gene clusters showing that members of these taxa should be given greater importance in the search for new antimicrobials and other industrially important biomolecules. The plasmid-borne operon encoding fossil fuel desulfurization enzymes was present in IGTS8 and XP suggesting that it might be transferable between members of these species.
菌株IGTS8是通过无损硫特异性4S途径对化石燃料进行生物脱硫研究最为广泛的模式细菌。该菌株最初被归类为 ，后来又被归类为 ，因此其分类地位存在争议，这也反映出当时可用方法的分辨率有限。在本研究中，对菌株IGTS8和密切相关的红球菌全基因组序列进行的系统基因组分析表明， 和 是非常密切相关的物种，菌株IGTS8是 菌株，并且一些被鉴定为 的菌株应重新分类为 。归属于这些物种的菌株基因组包含潜在的新型生物合成基因簇，这表明在寻找新的抗菌剂和其他工业上重要的生物分子时，这些分类群的成员应得到更大的重视。编码化石燃料脱硫酶的质粒携带的 操纵子存在于IGTS8和XP中，这表明它可能在这些物种的成员之间转移。
