Aiyegbusi A I, Duru Fio, Akinbo S R, Noronha C C, Okanlawon A O
Department of Anatomy and.
Department of Physiotherapy, College of Medicine, University of Lagos, Lagos, Nigeria.
Open Access Rheumatol. 2010 Aug 4;2:45-52. doi: 10.2147/oarrr.s12118. eCollection 2010.
This study investigated the effects of low- and high-intensity intrasound therapy (LITR and HITR, respectively) given once daily and twice daily on the morphology and oxidative stress in healing tendon tissue following an acute injury.
Eighty-five male rats, randomized into six groups were further subdivided into groups A, B, and C, except for Group 1 which was subdivided into A and B only. Groups 2-6 underwent an induced crush injury. The six groups were allocated to: serve as controls (Group 1), receive no treatment (Group 2), HITR twice daily (Group 3), HITR once daily (Group 4), LITR twice daily (Group 5), and LITR once daily (Group 6). Intrasound therapy (ITR) was commenced 24 hours postinjury and was given once daily or twice daily over the first 14 days postinjury. The animals in subgroups A and B were sacrificed on day 15 postinjury, and those in subgroup C were sacrificed on day 31 postinjury. The tendons were excised, and processed for histology and malondialdehyde (MDA) assay.
There was no significant difference in the tenocyte population between the HITR- and LITR-treated groups. However, twice-daily treatment in either the low- or high-intensity mode resulted in significant tenocyte proliferation compared with the once-daily treated groups, and also had the highest percentage of tenoblasts compared with the population of tenocytes in the proliferative phase of healing. All treatment protocols marginally lowered the MDA level.
The role of IRT in tendon healing is influenced more by the frequency of treatment rather than the intensity of the delivered dosage.
本研究调查了每日一次和每日两次给予的低强度和高强度超声治疗（分别为LITR和HITR）对急性损伤后愈合肌腱组织形态和氧化应激的影响。
85只雄性大鼠随机分为6组，除第1组仅分为A和B亚组外，其余2 - 6组再进一步细分为A、B和C亚组。第2 - 6组接受诱导挤压伤。6组分别为：作为对照组（第1组）、不接受治疗（第2组）、每日两次HITR（第3组）、每日一次HITR（第4组）、每日两次LITR（第5组）和每日一次LITR（第6组）。超声治疗（ITR）在损伤后24小时开始，在损伤后的前14天每日一次或每日两次给予。A和B亚组的动物在损伤后第15天处死，C亚组的动物在损伤后第31天处死。切除肌腱并进行组织学和丙二醛（MDA）测定。
HITR和LITR治疗组之间的腱细胞数量没有显著差异。然而，与每日一次治疗组相比，低强度或高强度模式下的每日两次治疗导致显著的腱细胞增殖，并且与愈合增殖期的腱细胞群体相比，成纤维细胞的百分比也最高。所有治疗方案均使MDA水平略有降低。
IRT在肌腱愈合中的作用更多地受治疗频率而非给药剂量强度的影响。
