Budiarto Bugi Ratno
Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Data Brief. 2016 Sep 24;9:689-695. doi: 10.1016/j.dib.2016.09.033. eCollection 2016 Dec.
The dataset presented in this article is related to the research article entitled "Detection of Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur in polymorphism detection. Combining a new set of primers with PCR gradient, The allele-specific PCR well performs to detect all type of breast cancer-originated genotypes. The validation of this method was done using Sanger DNA sequencing, offering an alternative tool for polymorphism detection in another type of cancer.
