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Dataset reporting detection of breast cancer-related polymorphism using allele-specific polymerase chain reaction.

作者信息

Budiarto Bugi Ratno

机构信息

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong 16911, Indonesia.

出版信息

Data Brief. 2016 Sep 24;9:689-695. doi: 10.1016/j.dib.2016.09.033. eCollection 2016 Dec.

Abstract

The dataset presented in this article is related to the research article entitled "Detection of Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur in polymorphism detection. Combining a new set of primers with PCR gradient, The allele-specific PCR well performs to detect all type of breast cancer-originated genotypes. The validation of this method was done using Sanger DNA sequencing, offering an alternative tool for polymorphism detection in another type of cancer.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0872/5072141/25bd1a49af92/gr1.jpg

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