伊朗复发缓解型多发性硬化症患者中CYP27B1基因表达的下调

Down-regulation of CYP27B1 gene expression in Iranian patients with relapsing-remitting multiple sclerosis.

作者信息

Rezaie Zahra, Taheri Mohammad, Kohan Leila, Sayad Arezou

机构信息

Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.

Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Hum Antibodies. 2016;24(3-4):71-76. doi: 10.3233/HAB-160297.

DOI:10.3233/HAB-160297

PMID:27792005

Abstract

BACKGROUND

Multiple sclerosis (MS) as a complex neurological disease can be due to vitamin D deficiency. CYP27B1 is referred to as a vitamin D metabolizing enzyme.

MATERIALS AND METHODS

This study compared the expression level of CYP27B1 in Relapsing-Remitting MS (RRMS) patients with normal individuals in Iran. The RNA was extracted from 50 RRMS patients and 50 normal controls. Quantitative RT-PCR was adopted to measure the expression level of CYP27B1 gene.

RESULTS

The expression level of CYP27B1gene was significantly lower in the RRMS patients than their normal counterparts (P value = 0.04). Also, the RRMS females participating had a significant reduction in CYP27B1 gene expression compared to normal females (P-Value = 0.01). In addition, the correlation between CYP27B1 expression level, and the risk of Expanded Disability Status Scale of Kurtzke (EDSS) was not linear. Additionally, there was no significant correlation between expression status of CYP27B1gene and duration of the disease.

CONCLUSION

A significant decrease in the expression level of CYP27A1 in female patients could indicate their greater vulnerability to MS than the male patients.

摘要

背景

多发性硬化症（MS）作为一种复杂的神经系统疾病，可能归因于维生素D缺乏。CYP27B1被称为一种维生素D代谢酶。

材料与方法

本研究比较了伊朗复发缓解型多发性硬化症（RRMS）患者与正常个体中CYP27B1的表达水平。从50例RRMS患者和50例正常对照中提取RNA。采用定量逆转录聚合酶链反应（qRT-PCR）来测量CYP27B1基因的表达水平。

结果

RRMS患者中CYP27B1基因的表达水平显著低于正常对照（P值 = 0.04）。此外，参与研究的RRMS女性患者与正常女性相比，CYP27B1基因表达显著降低（P值 = 0.01）。此外，CYP27B1表达水平与库尔特克扩展残疾状态量表（EDSS）风险之间的相关性并非呈线性。另外，CYP27B1基因的表达状态与疾病持续时间之间无显著相关性。