6丙型肝炎病毒基因分型9G检测及其与VERSANT丙型肝炎病毒基因分型2.0检测法（线性探针分析法）用于丙型肝炎病毒基因分型的比较。

6 HCV genotyping 9G test and its comparison with VERSANT HCV genotype 2.0 assay (LiPA) for the hepatitis C virus genotyping.

作者信息

Chantratita Wasun, Song Keum-Soo, GunHo Choi, Pongthanapisith Viroj, Thongbaiphet Nipa, Wongtabtim Garanyuta, Pasomsub Ekawat, Angkanavin Kanokwan, Nimse Satish Balasaheb, Sonawane Mukesh Digambar, Warkad Shrikant Dasharath, Kim Taisun

机构信息

Virology Laboratory, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.

Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 24252, Korea.

出版信息

J Virol Methods. 2017 Jan;239:1-8. doi: 10.1016/j.jviromet.2016.10.009. Epub 2016 Oct 25.

DOI:10.1016/j.jviromet.2016.10.009

PMID:27793646

Abstract

In this article, we describe the 6 HCV Genotyping 9G test and its evaluation by using clinical samples and plasmid DNA standards. In tests with 981 plasmid DNA standards, the 6 HCV Genotyping 9G test showed higher than 92.5% sensitivity and 99.4% specificity. The 6 HCV Genotyping 9G test was compared with the VERSANT HCV Genotype 2.0 assay (LiPA 2.0) for detection and discrimination of HCV genotypes in clinical samples. The results of both tests were verified by genomic sequencing. The 6 HCV Genotyping 9G test demonstrated a 100% agreement with the sequencing results, which was higher than LiPA 2.0. These results indicate that the 6 HCV Genotyping 9G test can be a reliable, sensitive, and accurate diagnostic tool for the correct identification of HCV genotypes in clinical specimens. 6 HCV Genotyping 9G test can genotype six HCV types in 1 PCR in 30min after PCR amplification. The 6 HCV Genotyping 9G test, thus provide critical information to physicians and assist them to apply accurate drug regimen for the effective hepatitis C treatment.

摘要

在本文中，我们描述了6 HCV基因分型9G检测方法及其使用临床样本和质粒DNA标准品进行的评估。在对981个质粒DNA标准品的检测中，6 HCV基因分型9G检测显示出高于92.5%的灵敏度和99.4%的特异性。将6 HCV基因分型9G检测与VERSANT HCV基因分型2.0检测法（线性探针分析2.0）进行比较，以检测和区分临床样本中的HCV基因型。两种检测的结果均通过基因组测序进行验证。6 HCV基因分型9G检测与测序结果显示100%一致，高于线性探针分析2.0。这些结果表明，6 HCV基因分型9G检测可以成为一种可靠、灵敏且准确的诊断工具，用于在临床标本中正确鉴定HCV基因型。6 HCV基因分型9G检测可在PCR扩增后30分钟内通过1次PCR对6种HCV类型进行基因分型。因此，6 HCV基因分型9G检测为医生提供关键信息，并协助他们应用准确的药物治疗方案以有效治疗丙型肝炎。