氟他胺对人涎腺导管癌细胞雄激素诱导的恶性表型的阻断作用
Blockade of Androgen-induced Malignant Phenotypes by Flutamide Administration in Human Salivary Duct Carcinoma Cells.
作者信息
Kamata Y U, Sumida Tomoki, Murase Ryuichi, Nakano Hiroyuki, Yamada Tomohiro, Mori Yoshihide
机构信息
Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
出版信息
Anticancer Res. 2016 Nov;36(11):6071-6075. doi: 10.21873/anticanres.11196.
BACKGROUND/AIM: Androgens are known to play a critical role in prostate cancer progression, but their effect on malignant phenotypes in salivary gland cancer is unclear. The androgen-androgen receptor (AR) axis may be involved in malignant phenotypes of salivary duct carcinoma (SDC) cells and therefore may be a new target for SDC treatment. To test this hypothesis, we investigated the effect of the androgen 5α-dihydrotestosterone (DHT) on proliferation, migration, and invasiveness of SDC cells.
MATERIALS AND METHODS
We used a wound-healing assay to measure cell migration and a Boyden chamber invasion assay to investigate SDC cell invasive capacity.
RESULTS
DHT treatment increased cell proliferation, migration, and invasion. However, treatment with flutamide, an AR inhibitor, blocked the effects of DHT.
CONCLUSION
These results suggest that the androgen-AR axis is involved in SDC malignancy and may be an effective therapeutic target for treatment of human SDC.
背景/目的：已知雄激素在前列腺癌进展中起关键作用，但它们对唾液腺癌恶性表型的影响尚不清楚。雄激素-雄激素受体（AR）轴可能参与涎腺导管癌（SDC）细胞的恶性表型，因此可能成为SDC治疗的新靶点。为验证这一假设，我们研究了雄激素5α-二氢睾酮（DHT）对SDC细胞增殖、迁移和侵袭能力的影响。
材料与方法
我们采用伤口愈合试验测量细胞迁移，并使用博伊登小室侵袭试验研究SDC细胞的侵袭能力。
结果
DHT处理可增加细胞增殖、迁移和侵袭。然而，使用AR抑制剂氟他胺处理可阻断DHT的作用。
结论
这些结果表明雄激素-AR轴参与SDC的恶性过程，可能是治疗人类SDC的有效治疗靶点。
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