Steen R G, Tamargo R J, Brem H, Glickson J D, Wehrle J P
Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Magn Reson Med. 1989 Aug;11(2):258-66. doi: 10.1002/mrm.1910110214.
The 9L gliosarcoma, grown subcutaneously in juvenile Fischer 344 rats, was studied by in vivo 31P NMR spectroscopy following treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea. Dose-dependent increases in the proportion of high-energy phosphates were observed for doses between 10 and 36 mg/kg (from 80% of the LD10 to greater than the LD50). These doses reduced clonogenic cell survival in a dose-dependent fashion by as much as 3 log orders and resulted in up to 16 days of growth delay (to pretreatment tumor volume). Increases in high-energy phosphates (relative to Pi) in the tumor were greater at higher doses despite the higher levels of clonogenic cell killing and the substantial host systemic toxicity.
在幼年费希尔344大鼠皮下生长的9L胶质肉瘤，在用1,3-双（2-氯乙基）-1-亚硝基脲治疗后，通过体内31P核磁共振波谱进行了研究。在10至36mg/kg的剂量范围内（从LD10的80%到大于LD50），观察到高能磷酸盐比例呈剂量依赖性增加。这些剂量以剂量依赖性方式使克隆形成细胞存活率降低多达3个对数级，并导致长达16天的生长延迟（至预处理肿瘤体积）。尽管克隆形成细胞杀伤水平较高且宿主全身毒性较大，但肿瘤中高能磷酸盐（相对于无机磷酸盐）的增加在较高剂量时更大。
