Steen R G, Tamargo R J, McGovern K A, Rajan S S, Brem H, Wehrle J P, Glickson J D
Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Cancer Res. 1988 Feb 1;48(3):676-81.
In vivo 31P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triphosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.
采用体内31P核磁共振波谱法，研究大鼠9L胶质肉瘤在未经治疗的生长过程中以及对1,3-双（2-氯乙基）-1-亚硝基脲化疗的反应中的生物能量学。肿瘤生长与磷酸肌酸和核苷三磷酸共振的下降有关，这与未经治疗的生长过程中肿瘤缺氧增加一致。用1,3-双（2-氯乙基）-1-亚硝基脲（10mg/kg）化疗后，肿瘤中的磷酸肌酸和核苷三磷酸水平反弹，而肿瘤中的无机磷酸盐水平下降。治疗后4天对治疗组和未治疗组肿瘤切片进行组织学比较，结果显示，与对照肿瘤相比，治疗组肿瘤坏死细胞比例较低，存活细胞比例较高，间质空间水平高5倍。
