Blose Kory J, Pichamuthu Joseph E, Weinbaum Justin S, Vorp David A
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA.
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA; Center for Vascular Remodeling and Regeneration, University of Pittsburgh, Pittsburgh, PA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA.
Soft Mater. 2016;14(2):72-77. doi: 10.1080/1539445X.2016.1141787. Epub 2016 Jan 21.
Current commercial tensile testing systems use spring-loaded or other compression-based grips to clamp materials in place posing a problem for very soft or delicate materials that cannot withstand this mechanical clamping force. In order to perform uniaxial tensile tests on soft tissues or materials, we have created a novel vacuum-assisted anchor (VAA). Fibrin gels were subjected to uniaxial extension, and the testing data was used to determine material mechanical properties. Utilizing the VAA, we achieved successful tensile breaks of soft fibrin gels while finding statistically significant differences between the mechanical properties of gels fabricated at two different fibrinogen concentrations.
目前的商业拉伸测试系统使用弹簧加载或其他基于压缩的夹具将材料固定在适当位置,这对于无法承受这种机械夹紧力的非常柔软或脆弱的材料来说是个问题。为了对软组织或材料进行单轴拉伸测试,我们创建了一种新型的真空辅助锚定器(VAA)。对纤维蛋白凝胶进行单轴拉伸,并利用测试数据来确定材料的力学性能。利用VAA,我们成功地使柔软的纤维蛋白凝胶发生拉伸断裂,同时发现两种不同纤维蛋白原浓度制备的凝胶在力学性能上存在统计学上的显著差异。