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小鼠胸苷酸合成酶启动子：关键元件紧邻转录起始位点。

The mouse thymidylate synthase promoter: essential elements are in close proximity to the transcriptional initiation sites.

作者信息

Deng T, Li Y, Jolliff K, Johnson L F

机构信息

Department of Biochemistry, Ohio State University, Columbus 43210.

出版信息

Mol Cell Biol. 1989 Sep;9(9):4079-82. doi: 10.1128/mcb.9.9.4079-4082.1989.

DOI:10.1128/mcb.9.9.4079-4082.1989
PMID:2779579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362476/
Abstract

The promoter region of the mouse thymidylate synthase gene was analyzed by deletion and site-directed mutagenesis. Elimination of an upstream Sp1 element reduced expression threefold, whereas elimination of an adenovirus upstream stimulatory factor element had little effect. All of the upstream elements that are essential for promoter activity are located within 22 nucleotides of the first transcriptional initiation site.

摘要

通过缺失和定点诱变分析了小鼠胸苷酸合成酶基因的启动子区域。去除上游的Sp1元件使表达降低了三倍，而去除腺病毒上游刺激因子元件则几乎没有影响。启动子活性所必需的所有上游元件都位于第一个转录起始位点的22个核苷酸范围内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f25/362476/100504738aa6/molcellb00057-0501-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f25/362476/13dda7e6e866/molcellb00057-0501-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f25/362476/100504738aa6/molcellb00057-0501-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f25/362476/13dda7e6e866/molcellb00057-0501-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f25/362476/100504738aa6/molcellb00057-0501-b.jpg

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本文引用的文献

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Cell cycle regulation of thymidylate synthetase gene expression in cultured mouse fibroblasts.培养的小鼠成纤维细胞中胸苷酸合成酶基因表达的细胞周期调控
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生长刺激细胞中小鼠胸苷酸合成酶基因表达的调控：上游S期控制元件与必需启动子元件难以区分。
Nucleic Acids Res. 1995 Nov 25;23(22):4649-56. doi: 10.1093/nar/23.22.4649.
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Lack of an initiator element is responsible for multiple transcriptional initiation sites of the TATA-less mouse thymidylate synthase promoter.缺乏起始元件是无TATA盒的小鼠胸苷酸合成酶启动子存在多个转录起始位点的原因。
Mol Cell Biol. 1993 Aug;13(8):4894-903. doi: 10.1128/mcb.13.8.4894-4903.1993.
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Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence.内含子对基因表达的刺激作用：通过改变剪接供体序列将抑制性内含子转变为刺激性内含子。
Nucleic Acids Res. 1993 Dec 25;21(25):5901-8. doi: 10.1093/nar/21.25.5901.
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Cloning, chromosomal location, and characterization of mouse E2F1.小鼠E2F1的克隆、染色体定位及特性分析
Mol Cell Biol. 1994 Mar;14(3):1861-9. doi: 10.1128/mcb.14.3.1861-1869.1994.
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Introns are essential for growth-regulated expression of the mouse thymidylate synthase gene.内含子对于小鼠胸苷酸合成酶基因的生长调节表达至关重要。
Mol Cell Biol. 1993 Mar;13(3):1565-71. doi: 10.1128/mcb.13.3.1565-1571.1993.
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Bidirectional promoter of the mouse thymidylate synthase gene.小鼠胸苷酸合成酶基因的双向启动子。
Nucleic Acids Res. 1994 Oct 11;22(20):4044-9. doi: 10.1093/nar/22.20.4044.
9
Multiple protein-DNA interactions in the TATAA-less mouse thymidylate synthase promoter.无TATA盒的小鼠胸苷酸合成酶启动子中的多种蛋白质-DNA相互作用。
Nucleic Acids Res. 1991 May 11;19(9):2267-74. doi: 10.1093/nar/19.9.2267.
10
The 5'-flanking region of the mouse thymidylate synthase gene is necessary but not sufficient for normal regulation in growth-stimulated cells.小鼠胸苷酸合成酶基因的5'侧翼区域对于生长刺激细胞的正常调控是必要的，但并不充分。
Mol Cell Biol. 1991 Feb;11(2):1023-9. doi: 10.1128/mcb.11.2.1023-1029.1991.
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The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.启动子特异性转录因子Sp1与SV40早期启动子中的上游序列结合。
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J Biol Chem. 1984 Jun 10;259(11):7206-11.
8
Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.寡核苷酸定向诱变：一种使用两条寡核苷酸引物和单链DNA模板的简单方法。
DNA. 1984 Dec;3(6):479-88. doi: 10.1089/dna.1.1984.3.479.
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An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter.一种RNA聚合酶II转录因子与腺病毒主要晚期启动子中的上游元件结合。
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