Changes in Expression of Connexin Isoforms in the Caudal Epididymis of Adult Sprague-Dawley Rats exposed to Estradiol Benzoate or Flutamide at the Neonatal Age.

Direct communication between neighboring cells via gap junction in tissue is important for maintenance and regulation of its physiological functions. Each epididymal region has different composition of cell types. It is well recognized that the epididymis is a steroid hormone-responsive tissue. The present study was designed to determine the effect of estradiol benzoate (EB) or flutamide exposured at the early postnatal age on the expression of connexin () isoforms in the caudal epididymis. The EB or flutamide was subcutaneously administrated to male Spragure Dawley rat at 7 days of age, and expressional changes of isoforms in the adult corpus epididymis were determined by quantitative real-time PCR. The treatment of low-dose EB resulted in decreases of 30.3, 31.1, 37, and 45 expression but caused an increase of 32 expression. Exposure to high-dose EB led into expressional increases of 31, 31.1, 32, 40, and 43, even though a decrease of 37 expression was found with a high-dose EB treatment. A low-dose flutamide induced increases of 31, 31.1, 32, and 43 expression but a decrease of 37 expression. Expression of most genes were significantly increased by a high-dose flutamide, while no expressional change of 26 and 40 was detected by a high-dose flutamide. These results indicate that expression of isoforms in the caudal epididymis is altered by exposure to steroidal compounds at the prepubertal age. It is suggested that a contact with environmental exogenous materials during the early postnatal period would lead to alteration of epididymal functions at the adult.