Rezende Jorge A M, Camelo-García Viviana M, Andrade Sónia C S, Buriolla José E, Kitajima Elliot W, Duarte Ligia M L
Departamento de Fitopatologia e Nematologia, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.
Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Arch Virol. 2017 Feb;162(2):529-533. doi: 10.1007/s00705-016-3114-2. Epub 2016 Oct 28.
In this work, we report the complete genome sequence of, production of polyclonal antibodies against, and development of biological assays for a putative new potexvirus, named senna mosaic virus (SenMV), found infecting Senna occidentalis in the state of São Paulo, Brazil. The complete genome sequence of SenMV comprises 6775 nucleotides excluding the poly(A) tail. The genome organization is similar to those of other potexviruses, with five open reading frames coding for RNA-dependent RNA polymerase (RdRp), the triple gene block (TGB 1, 2, and 3) proteins, and coat protein (CP). The virus was transmitted to S. occidentalis by mechanical inoculation and trimming scissors, but not by seeds.
在本研究中，我们报告了一种假定的新型马铃薯X病毒（命名为番泻叶花叶病毒，简称SenMV）的全基因组序列，针对该病毒生产了多克隆抗体，并开发了生物学检测方法。该病毒是在巴西圣保罗州发现的，感染了西番莲。SenMV的全基因组序列（不包括聚腺苷酸尾）由6775个核苷酸组成。其基因组结构与其他马铃薯X病毒相似，有五个开放阅读框，分别编码依赖RNA的RNA聚合酶（RdRp）、三基因块（TGB 1、2和3）蛋白以及外壳蛋白（CP）。该病毒通过机械接种和修剪剪刀传播给西番莲，但不能通过种子传播。
