Yang Bing-Bing, Chen Yuan-Hua, Zhang Cheng, Shi Chang-E, Hu Kai-Feng, Zhou Ju, Xu De-Xiang, Chen Xi
Department of Gastroenterology, Fourth Affiliated Hospital of Anhui Medical University, Hefei, 230032, China.
Department of Toxicology, Anhui Medical University, Hefei, 230032, China.
Endocrine. 2017 Feb;55(2):582-590. doi: 10.1007/s12020-016-1152-x. Epub 2016 Oct 31.
Several studies explored the association between vitamin D status and nonalcoholic fatty liver disease with contradictory results. We aimed to investigate the association between vitamin D status, inflammatory cytokines and liver fibrosis in nonalcoholic fatty liver disease patients. Two hundred nineteen nonalcoholic fatty liver disease patients and 166 age- and gender- matched healthy controls were recruited for this study. Serum 25(OH)D was measured by radioimmunoassay. Serum interleukin-8 and transforming growth factor-β1 were measured using ELISA. Serum 25(OH)D was only marginally decreased in nonalcoholic fatty liver disease patients. Interestingly, serum 25(OH)D was markedly reduced in nonalcoholic fatty liver disease patients with advanced liver fibrosis compared to nonalcoholic fatty liver disease patients with indeterminate liver fibrosis and no advanced fibrosis. Logistic regression analysis showed that there was an inverse association between serum 25(OH)D and severity of liver fibrosis in nonalcoholic fatty liver disease patients. Further analysis showed that serum interleukin-8 was elevated in nonalcoholic fatty liver disease patients, the highest interleukin-8 in patients with advanced fibrosis. An inverse correlation between serum 25(OH)D and interleukin-8 was observed in nonalcoholic fatty liver disease patients with and without liver fibrosis. Although serum transforming growth factor-β1 was slightly elevated in nonalcoholic fatty liver disease patients, serum transforming growth factor-β1 was reduced in nonalcoholic fatty liver disease patients with advanced fibrosis. Unexpectedly, a positive correlation between serum 25(OH)D and transforming growth factor-β1 was observed in nonalcoholic fatty liver disease patients with advanced fibrosis. In conclusion, low vitamin D status is associated with advanced liver fibrosis in nonalcoholic fatty liver disease patients. Interleukin-8 may be an important mediator for hepatic fibrosis in nonalcoholic fatty liver disease patients with low vitamin D status.
多项研究探讨了维生素D状态与非酒精性脂肪性肝病之间的关联，但结果相互矛盾。我们旨在研究非酒精性脂肪性肝病患者的维生素D状态、炎性细胞因子与肝纤维化之间的关联。本研究招募了219例非酒精性脂肪性肝病患者和166例年龄及性别匹配的健康对照。采用放射免疫分析法测定血清25(OH)D。使用酶联免疫吸附测定法测定血清白细胞介素-8和转化生长因子-β1。非酒精性脂肪性肝病患者的血清25(OH)D仅略有下降。有趣的是，与肝纤维化程度不确定且无晚期纤维化的非酒精性脂肪性肝病患者相比，晚期肝纤维化的非酒精性脂肪性肝病患者血清25(OH)D明显降低。逻辑回归分析显示，非酒精性脂肪性肝病患者血清25(OH)D与肝纤维化严重程度呈负相关。进一步分析显示，非酒精性脂肪性肝病患者血清白细胞介素-8升高，晚期纤维化患者的白细胞介素-8最高。在有和没有肝纤维化的非酒精性脂肪性肝病患者中均观察到血清25(OH)D与白细胞介素-8呈负相关。虽然非酒精性脂肪性肝病患者血清转化生长因子-β1略有升高，但晚期纤维化的非酒精性脂肪性肝病患者血清转化生长因子-β1降低。出乎意料的是，在晚期纤维化的非酒精性脂肪性肝病患者中观察到血清25(OH)D与转化生长因子-β1呈正相关。总之，维生素D水平低与非酒精性脂肪性肝病患者的晚期肝纤维化有关。白细胞介素-8可能是维生素D水平低的非酒精性脂肪性肝病患者肝纤维化的重要介质。
Zotero 插件