Kumsaen Piyawan, Fucharoen Goonnapa, Sirijerachai Chittima, Chainansamit Su-On, Wisanuyothin Nittaya, Kuwatjanakul Pichayanan, Wiangnon Surapon
The Medical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand E-mail :
Asian Pac J Cancer Prev. 2016;17(9):4395-4399.
The FLT3-ITD mutation is one of the most frequent genetic abnormalities in acute myeloid leukemia (AML) where it is associated with a poor prognosis. The FLT3-ITD mutation could, therefore, be a potential molecular prognostic marker important for risk-stratified treatment options. We amplified the FLT3 gene at exon 14 and 15 in 52 AML patients (aged between 2 months and 74 years) from 4 referral centers (a university hospital and 3 regional hospitals in Northeast Thailand), using a simple PCR method. FLT3-ITD mutations were found in 10 patients (19.2%), being more common in adults than in children (21.1% vs. 14.3%) and more prevalent in patients with acute promyelocytic leukemia (AML-M3) than AML-non M3 (4 of 10 AML-M3 vs. 6 of 42 AML- non M3 patients). Duplication sequences varied in size-between 27 and 171 nucleotides (median=63.5) and in their location. FLT3-ITD mutations with common duplication sequences accounted for a significant percentage in AML patients in northeastern Thailand. This simple PCR method is feasible for routine laboratory practice and these data could help tailor use of the national protocol for AML.
FLT3-ITD突变是急性髓系白血病（AML）中最常见的基因异常之一，与预后不良相关。因此，FLT3-ITD突变可能是一个潜在的分子预后标志物，对风险分层治疗方案很重要。我们采用简单的聚合酶链反应（PCR）方法，对来自泰国东北部4个转诊中心（1所大学医院和3所地区医院）的52例AML患者（年龄在2个月至74岁之间）的FLT3基因第14和15外显子进行了扩增。在10例患者（19.2%）中发现了FLT3-ITD突变，在成人中比儿童更常见（21.1%对14.3%），在急性早幼粒细胞白血病（AML-M3）患者中比非M3型AML更普遍（10例AML-M3患者中有4例，42例非M3型AML患者中有6例）。重复序列的大小在27至171个核苷酸之间变化（中位数=63.5），其位置也不同。具有常见重复序列的FLT3-ITD突变在泰国东北部的AML患者中占相当大的比例。这种简单的PCR方法适用于常规实验室操作，这些数据有助于调整AML国家方案的使用。
