Presentini Rivo
a Sclavo Diagnostics International Srl, Protein Development and Quality Control Department , Siena , Italy.
J Immunoassay Immunochem. 2017;38(1):100-113. doi: 10.1080/15321819.2016.1250773. Epub 2016 Oct 31.
A new method has been developed to prepare horseradish peroxidase (HRP) conjugates using bis(sulfosuccinimidyl)suberate (BS3) as cross-linking reagent in a two-step procedure. The enzyme is reacted with BS3, introducing active ester molecules into the enzyme itself, and it is then used directly to label amino-containing compounds without further treatment. The proteins involved in the conjugation undergo minimal modifications. The reaction conditions are evaluated, as well as studies on the conservation of the biological activities of the conjugated proteins and the stability of the conjugates in time. These conjugates are found to be significantly improved compared with similar products prepared by conventional methods.
已开发出一种新方法，分两步使用双（磺基琥珀酰亚胺）辛二酸酯（BS3）作为交联剂来制备辣根过氧化物酶（HRP）缀合物。酶与BS3反应，将活性酯分子引入酶本身，然后直接用于标记含氨基化合物，无需进一步处理。参与缀合的蛋白质经历的修饰最小。评估了反应条件，以及对缀合蛋白质生物活性的保留和缀合物随时间的稳定性进行了研究。发现这些缀合物与通过传统方法制备的类似产品相比有显著改进。
