Niemirowicz Katarzyna, Piktel Ewelina, Wilczewska Agnieszka Z, Markiewicz Karolina H, Durnaś Bonita, Wątek Marzena, Puszkarz Irena, Wróblewska Marta, Niklińska Wiesława, Savage Paul B, Bucki Robert
Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok.
Institute of Chemistry, University of Białystok, Białystok.
Int J Nanomedicine. 2016 Oct 19;11:5443-5455. doi: 10.2147/IJN.S113706. eCollection 2016.
Core-shell magnetic nanoparticles (MNPs) are promising candidates in the development of new treatment methods against infections, including those caused by antibiotic-resistant pathogens. In this study, the bactericidal activity of human antibacterial peptide cathelicidin LL-37, synthetic ceragenins CSA-13 and CSA-131, and classical antibiotics vancomycin and colistin, against methicillin-resistant Xen 30 and Xen 5, was assessed alone and in combination with core-shell MNPs. Fractional inhibitory concentration index and fractional bactericidal concentration index were determined by microdilution methods. The potential of combined therapy using nanomaterials and selected antibiotics was confirmed using chemiluminescence measurements. Additionally, the ability of tested agents to prevent bacterial biofilm formation was evaluated using crystal violet staining. In most conditions, synergistic or additive effects were observed when combinations of core-shell MNPs with ceragenins or classical antibiotics were used. Our study revealed that a mixture of membrane-active agents such as LL-37 peptide or ceragenin CSA-13 with MNPs potentialized their antibacterial properties and might be considered as a method of delaying and overcoming bacterial drug resistance.
核壳磁性纳米颗粒（MNPs）是开发针对感染（包括由抗生素耐药病原体引起的感染）的新治疗方法的有前景的候选材料。在本研究中，评估了人抗菌肽cathelicidin LL-37、合成杀菌素CSA-13和CSA-131以及经典抗生素万古霉素和多粘菌素对耐甲氧西林的Xen 30和Xen 5的杀菌活性，单独使用以及与核壳MNPs联合使用时的情况。通过微量稀释法测定部分抑菌浓度指数和部分杀菌浓度指数。使用化学发光测量法证实了使用纳米材料和选定抗生素联合治疗的潜力。此外，使用结晶紫染色评估了受试药物预防细菌生物膜形成的能力。在大多数情况下，当使用核壳MNPs与杀菌素或经典抗生素联合使用时，观察到协同或相加作用。我们的研究表明，膜活性剂如LL-37肽或杀菌素CSA-13与MNPs的混合物增强了它们的抗菌性能，可被视为一种延缓和克服细菌耐药性的方法。
