文库定量：使用Qubit系统对双链或单链DNA样本进行荧光定量。
Library Quantification: Fluorometric Quantitation of Double-Stranded or Single-Stranded DNA Samples Using the Qubit System.
作者信息
Mardis Elaine, McCombie W Richard
出版信息
Cold Spring Harb Protoc. 2017 Jun 1;2017(6):pdb.prot094730. doi: 10.1101/pdb.prot094730.
PMID:27803271
Abstract
Qubit is an accurate and highly sensitive fluorescence-based quantitation system. Several assay kits have been optimized for the Qubit fluorometer, but also function efficiently with other fluorometers. The high-sensitivity dsDNA Qubit Kit has a detection range of 0.2-100 ng. The ssDNA Kit has a detection range of 1-200 ng.
摘要
Qubit是一种基于荧光的精确且高度灵敏的定量系统。有几种检测试剂盒已针对Qubit荧光计进行了优化，但也能与其他荧光计高效配合使用。高灵敏度双链DNA Qubit试剂盒的检测范围为0.2 - 100 ng。单链DNA试剂盒的检测范围为1 - 200 ng。
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