Kim Ki Taek, Lee Jae-Young, Park Ju-Hwan, Kim Min Hwan, Kim Ji-Su, Shin Hyeon-Jong, Kang Naewon, Cho Hyun-Jong, Yoon In-Soo, Kim Dae-Duk
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University.
Chem Pharm Bull (Tokyo). 2016;64(11):1582-1588. doi: 10.1248/cpb.c16-00405.
A simple and sensitive analytical method for the quantitative determination of buspirone in rat plasma by HPLC with fluorescence detection was developed and validated using naproxen as an internal standard. A relatively small-volume (150 µL) aliquot of rat plasma sample was prepared by a simple deproteinization procedure using acetonitrile as a precipitating organic solvent. Chromatographic separation was performed using Kinetex C8 column with an isocratic mobile phase consisting of acetonitrile and 10-mM potassium phosphate buffer (pH 6.0) at a flow rate of 1.0 mL/min. The eluent was monitored by fluorescence detector at a wavelength pair of 237/380 nm (excitation/emission). The linearity was established at 20.0-5000 ng/mL, and the limit of detection was 6.51 ng/mL. The precision (≤14.6%), accuracy (89.2-108%), and stability (89.1-101%) were within acceptable ranges. The newly developed method was successfully applied to intravenous and oral pharmacokinetic studies of buspirone in rats.
建立了一种采用高效液相色谱-荧光检测法测定大鼠血浆中丁螺环酮含量的简单灵敏的分析方法，并以萘普生作为内标进行了方法验证。通过使用乙腈作为沉淀有机溶剂的简单去蛋白程序，制备了相对小体积（150 μL）的大鼠血浆样品等分试样。使用Kinetex C8柱进行色谱分离，流动相为乙腈和10 mM磷酸钾缓冲液（pH 6.0）的等度洗脱，流速为1.0 mL/min。通过荧光检测器在237/380 nm（激发/发射）波长对下监测洗脱液。线性范围为20.0 - 5000 ng/mL，检测限为6.51 ng/mL。精密度（≤14.6%）、准确度（89.2 - 108%）和稳定性（89.1 - 101%）均在可接受范围内。新开发的方法成功应用于丁螺环酮在大鼠体内的静脉注射和口服药代动力学研究。
