Hur Justine Claire, Blaise Régis, Limon Isabelle
UPMC Univ Paris 06, Sorbonne Universités.
UPMC Univ Paris 06, Sorbonne Universités;
J Vis Exp. 2016 Oct 22(116):54352. doi: 10.3791/54352.
The cerebral arterial circle (circulus arteriosus cerebri) or circle of Willis (CoW) is a circulatory anastomosis surrounding the optic chiasma and hypothalamus that supplies blood to the brain and surrounding structures. It has been implicated in several cerebrovascular disorders, including cerebral amyloid angiopathy (CAA)-associated vasculopathies, intracranial atherosclerosis and intracranial aneurysms. Studies of the molecular mechanisms underlying these diseases for the identification of novel drug targets for their prevention require animal models. Some of these models may be transgenic, whereas others will involve isolation of the cerebro-vasculature, including the CoW.The method described here is suitable for CoW isolation in any mouse lineage and has considerable potential for screening (expression of genes, protein production, posttranslational protein modifications, secretome analysis, etc.) studies on the large vessels of the mouse cerebro-vasculature. It can also be used for ex vivo studies, by adapting the organ bath system developed for isolated mouse olfactory arteries.
大脑动脉环（脑动脉环）或Willis环（CoW）是围绕视交叉和下丘脑的循环吻合，为大脑和周围结构供血。它与多种脑血管疾病有关，包括脑淀粉样血管病（CAA）相关的血管病变、颅内动脉粥样硬化和颅内动脉瘤。研究这些疾病的潜在分子机制以确定预防的新药物靶点需要动物模型。其中一些模型可能是转基因的，而其他模型则涉及脑血管系统的分离，包括Willis环。这里描述的方法适用于任何小鼠品系的Willis环分离，并且在筛选（基因表达、蛋白质产生、蛋白质翻译后修饰、分泌组分析等）小鼠脑血管系统大血管的研究方面具有相当大的潜力。通过改编为分离的小鼠嗅动脉开发的器官浴系统，它还可用于离体研究。
