甲硫氨酸合成酶（MTR）和甲硫氨酸合成酶还原酶（MTRR）基因多态性在子痫前期发病机制中的作用
The polymorphisms of methionine synthase (MTR) and methionine synthase reductase (MTRR) genes in pathogenesis of preeclampsia.
作者信息
Seremak-Mrozikiewicz Agnieszka, Bogacz Anna, Deka-Pawlik Donata, Klejewski Andrzej, Wolski Hubert, Drews Krzysztof, Karasiewicz Monika, Czerny Boguslaw
机构信息
a Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences , Poznan , Poland.
b Department of Pharmacology and Phytochemistry.
出版信息
J Matern Fetal Neonatal Med. 2017 Oct;30(20):2498-2504. doi: 10.1080/14767058.2016.1254183. Epub 2017 Feb 5.
PURPOSE
The aim of the study was to determine the MTR (methionine synthase) and MTRR (mehionine synthase reductase) polymorphisms in pregnant women with preeclampsia (PE).
MATERIALS AND METHODS
The group of 98 women with PE and the group of 120 healthy pregnant women were analyzed. Determination of MTR 2756A > G and MTRR 66A > G polymorphisms was performed using polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP) method.
RESULTS
The study did not show any statistically significant differences in frequency of genotypes and alleles of MTR 2756A > G polymorphism between PE group and controls. Higher frequency of 66GG genotype and 66G allele of MTRR 66A > G polymorphism was observed in the women with PE compared to control group. Moreover, the 66GG genotype correlated with higher doses of methyldopa, lower birth weight and higher placenta weight in women with PE.
CONCLUSIONS
The obtained results for 66A > G polymorphism of MTRR gene suggest the predisposition to PE in carriers of mutated 66GG genotype and - 66G allele.
目的
本研究旨在确定子痫前期（PE）孕妇的甲硫氨酸合成酶（MTR）和甲硫氨酸合成酶还原酶（MTRR）基因多态性。
材料与方法
分析了98例PE孕妇和120例健康孕妇。采用聚合酶链反应（PCR）/限制性片段长度多态性（RFLP）方法测定MTR 2756A＞G和MTRR 66A＞G基因多态性。
结果
研究未显示PE组与对照组之间MTR 2756A＞G基因多态性的基因型和等位基因频率存在任何统计学显著差异。与对照组相比，PE组女性中观察到MTRR 66A＞G基因多态性的66GG基因型和66G等位基因频率更高。此外，66GG基因型与PE女性更高剂量的甲基多巴、更低的出生体重和更高的胎盘重量相关。
结论
MTRR基因66A＞G多态性的研究结果表明，携带突变的66GG基因型和-66G等位基因的个体易患PE。
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