Li Wei, Han Yunsheng, Yuan Xue, Wang Guan, Wang Zhibo, Pan Qiqi, Gao Yan, Qu Yongli
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Street, Daqing, 163319, Heilongjiang, China.
Arch Microbiol. 2017 Apr;199(3):433-443. doi: 10.1007/s00203-016-1311-8. Epub 2016 Nov 2.
Milk containing antibiotics is used as cost-effective feed for calves, which may lead to antibiotic residues-associated food safety problems. This study aims to investigate the influence of antibiotics on rumen microbes. Through metagenomic sequencing, the rumen microbial communities of calves fed with pasteurized milk containing antibiotics (B1), milk containing antibiotics (B2) and fresh milk (B3) were explored. Each milk group included calves in 2 (T1), 3 (T2) and 6 (T3) months of age. Using FastQC software and SOAPdenovo 2, the filtered data, respectively, were performed with quality control and sequence splicing. Following KEGG annotation was conducted for the uploaded sequences using KAAS software. Using R software, both species abundance analysis and differential abundance analysis were performed. In the B1 samples, the species abundance of Bacteroidetes gradually decreased along with the extension of feeding time, while that of Fibrobacteres gradually increased. The species abundances of Proteobacteria (p value = 0.01) and Spirochaetes (p value = 0.03) had significant differences among T1, T2 and T3 samples. Meanwhile, only the species abundance of Spirochaetes (p value = 0.04) had significant difference among B1, B2 and B3 samples. Cell cycle involving GSK3β, CDK2 and CDK7 was significantly enriched for the differentially expressed genes in the T1 versus T2 and T1 versus T3 comparison groups. Milk containing antibiotics might have a great influence on these rumen microbes and lead to antibiotic residues-associated food safety problems. Furthermore, GSK3β, CDK2 and CDK7 in rumen bacteria might affect milk fat metabolism in early growth stages of calves.
含有抗生素的牛奶被用作犊牛具有成本效益的饲料，这可能会导致与抗生素残留相关的食品安全问题。本研究旨在调查抗生素对瘤胃微生物的影响。通过宏基因组测序，探究了用含有抗生素的巴氏杀菌牛奶（B1）、含有抗生素的牛奶（B2）和新鲜牛奶（B3）喂养的犊牛的瘤胃微生物群落。每个牛奶组包括2月龄（T1）、3月龄（T2）和6月龄（T3）的犊牛。使用FastQC软件和SOAPdenovo 2分别对过滤后的数据进行质量控制和序列拼接。使用KAAS软件对上传的序列进行KEGG注释。使用R软件进行物种丰度分析和差异丰度分析。在B1样本中，拟杆菌门的物种丰度随着喂养时间的延长逐渐降低，而纤维杆菌门的物种丰度逐渐增加。变形菌门（p值 = 0.01）和螺旋体门（p值 = 0.03）的物种丰度在T1、T2和T3样本之间存在显著差异。同时，仅螺旋体门的物种丰度（p值 = 0.04）在B1、B2和B3样本之间存在显著差异。在T1与T2以及T1与T3比较组中，涉及GSK3β、CDK2和CDK7的细胞周期在差异表达基因中显著富集。含有抗生素的牛奶可能会对这些瘤胃微生物产生很大影响，并导致与抗生素残留相关的食品安全问题。此外，瘤胃细菌中的GSK3β、CDK2和CDK7可能会影响犊牛早期生长阶段的乳脂肪代谢。
