Postgraduate Program in Endodontics, European University of Madrid, Madrid, Spain.
Faculty of Health Sciences, School of Dentistry, The University of Adelaide, Adelaide, SA, Australia.
Int Endod J. 2017 Oct;50(10):976-982. doi: 10.1111/iej.12717. Epub 2016 Dec 3.
To compare soft-tissue dissolution by sodium hypochlorite, with an EDTA intermediate rinse, with or without activation with passive ultrasonic activation (PUI) or sonic activation using the Endoactivator (EA) or Eddy tips (ED).
The root canals of eighty-three human maxillary central incisors were chemo-mechanically prepared and the teeth split. A standardized longitudinal intracanal groove was created in one of the root halves. Eighty-three porcine palatal mucosa samples were collected, adapted to fit into the grooves and weighed. The re-assembled specimens were randomly divided into four experimental groups (n = 20), based on the final rinse: no activation; EA; PUI; ED, using 2.5% sodium hypochlorite, with an EDTA intermediate rinse. A control group (n = 3) was irrigated with distilled water without activation. The solutions were delivered using a syringe and needle 2 mm from working length. Total irrigation time was 150 s, including 60 s of activation in the specific groups. The study was carried out at 36 ± 2 °C. The porcine palatal mucosa samples were weighed after completion of the assays. Student paired t-test and anova were used to assess the intra- and intergroup weight changes. The multiple comparisons were evaluated using Bonferroni correction (α = 0.05).
Weight loss occurred in all experimental groups. Irrigant activation resulted in greater weight loss when compared to the nonactivated group [vs. EA (P = 0.001); vs. PUI (P < 0.001); vs. ED (P < 0.001)]. No significant differences were found amongst the different activation systems.
Activation increased the tissue-dissolving activity of irrigants from artificial grooves in root canals of maxillary central incisors.
比较次氯酸钠的软组织溶解作用，与 EDTA 中间冲洗，是否使用被动超声激活（PUI）或使用 Endoactivator（EA）或 Eddy 尖端（ED）进行的声激活进行激活。
用化学机械法预备 83 个人上颌中切牙的根管，并将牙齿劈开。在其中一个根半部分创建了一个标准化的纵向腔内槽。收集了 83 个猪腭黏膜样本，适应并适合放入槽中并称重。重新组装的标本根据最终冲洗分为四个实验组（n=20）：无激活；EA；PUI；ED，使用 2.5%次氯酸钠，并用 EDTA 中间冲洗。对照组（n=3）用无激活的蒸馏水冲洗。溶液通过注射器和针头从工作长度 2mm 处输送。总冲洗时间为 150s，包括特定组中 60s 的激活。研究在 36±2°C 进行。完成测定后，对猪腭黏膜样本进行称重。使用学生配对 t 检验和方差分析评估组内和组间的重量变化。使用 Bonferroni 校正（α=0.05）评估多重比较。
所有实验组均出现重量损失。与非激活组相比，冲洗激活导致更大的重量损失[与 EA（P=0.001）相比；与 PUI（P<0.001）相比；与 ED（P<0.001）相比]。不同的激活系统之间没有发现显著差异。
激活增加了人工槽中上颌中切牙根管中冲洗剂的组织溶解活性。
