利用多功能树枝状聚合物包裹的金纳米颗粒将治疗性小干扰RNA高效递送至胶质母细胞瘤细胞
Efficient delivery of therapeutic siRNA into glioblastoma cells using multifunctional dendrimer-entrapped gold nanoparticles.
作者信息
Kong Lingdan, Wu Yilun, Alves Carla S, Shi Xiangyang
机构信息
State Key Laboratory for Modification of Chemical Fibers & Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.
CQM-Centro de Quimica da Madeira, Universidade da Madeira, Campus da Penteada, 9000-390 Funchal, Portugal.
出版信息
Nanomedicine (Lond). 2016 Dec;11(23):3103-3115. doi: 10.2217/nnm-2016-0240. Epub 2016 Nov 4.
AIM
To synthesize the arginine-glycine-aspartic (RGD) functionalized dendrimer-entrapped gold nanoparticles (Au DENPs) for siRNA delivery to induce gene silencing of cancer cells in vitro and in vivo.
MATERIALS & METHODS: Au DENPs modified with RGD peptide via a polyethylene glycol spacer were used as a vector of two distinct small interfering RNAs (siRNAs) (VEGFvascular endothelial growth factor siRNA and B-cell lymphoma/leukemia-2 siRNA), and the physicochemical properties, cytocompatibility and transfection efficiency of Au DENP/siRNA polyplexes were characterized.
RESULTS
The Au DENP/siRNA polyplexes with good cytocompatibility and highly efficient transfection capacity can be used for the transfection of siRNAs.
CONCLUSION
The developed functional RGD-modified Au DENPs may be used for efficient gene therapy of different types of cancer.
目的
合成精氨酸 - 甘氨酸 - 天冬氨酸（RGD）功能化的树枝状聚合物包裹金纳米颗粒（Au DENPs），用于在体外和体内递送小干扰RNA（siRNA）以诱导癌细胞基因沉默。
材料与方法
通过聚乙二醇间隔基用RGD肽修饰的Au DENPs用作两种不同小干扰RNA（siRNA）（血管内皮生长因子（VEGF）小干扰RNA和B细胞淋巴瘤/白血病-2小干扰RNA）的载体，并对Au DENP/siRNA复合物的物理化学性质、细胞相容性和转染效率进行了表征。
结果
具有良好细胞相容性和高效转染能力的Au DENP/siRNA复合物可用于siRNA的转染。
结论
所开发的功能性RGD修饰的Au DENPs可用于不同类型癌症的高效基因治疗。
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