Conradi N G, Sjöström A
Department of Pathology, Gothenburg University, Sweden.
Acta Physiol Scand. 1989 Aug;136(4):589-96. doi: 10.1111/j.1748-1716.1989.tb08706.x.
Lamination of activity of two mitochondrial enzymes-succinate dehydrogenase (SDH) and menadione-dependent alpha-glycerophosphate dehydrogenase (M-GPDH) - was examined in the visual cortex of control (C) and protein-deprived (PD) rats from 10 days old to adult. In C rats, lamination of enzyme activity was evident from 10 days by a band of higher activity in lamina IV. A band of higher SDH activity was seen in superficial lamina VI from 15 days. Lamination of M-GPDH activity became less pronounced with increasing age, whereas distinct bands of high SDH activity in lamina IV and VI remained also in adult rats. PD rats showed a developmental delay of approximately 5 days with regard to the general increase in neuropile activity. A band of higher enzyme activity in lamina IV was present from 10 days, but it was markedly less distinct in the PD rats compared to the C rats between 15 and 25 days. A band of higher SDH activity in superficial lamina VI was not seen until 25 days in PD rats. No apparent differences were seen between adult C and PD rats. The observed developmental alterations are discussed in relation to other delays, distortions and deficits found in the visual system of PD rats.
在10日龄至成年的对照（C）大鼠和蛋白质缺乏（PD）大鼠的视皮层中，研究了两种线粒体酶——琥珀酸脱氢酶（SDH）和甲萘醌依赖性α-甘油磷酸脱氢酶（M-GPDH）活性的分层情况。在C大鼠中，从10日龄开始，酶活性分层就很明显，IV层有一条较高活性带。从15日龄开始，VI层浅部可见一条较高的SDH活性带。随着年龄增长，M-GPDH活性的分层变得不那么明显，而IV层和VI层中明显的高SDH活性带在成年大鼠中也依然存在。PD大鼠在神经毡活性总体增加方面表现出约5天的发育延迟。从10日龄开始，IV层就有一条较高的酶活性带，但在15至25日龄期间，与C大鼠相比，PD大鼠中的这条带明显不那么明显。PD大鼠直到25日龄才在VI层浅部看到一条较高的SDH活性带。成年C大鼠和PD大鼠之间未观察到明显差异。结合在PD大鼠视觉系统中发现的其他延迟、扭曲和缺陷，对观察到的发育变化进行了讨论。
