Juhlin C, Rastad J, Klareskog L, Grimelius L, Akerström G
Department of Surgery, University of Uppsala, Sweden.
Am J Pathol. 1989 Aug;135(2):321-8.
Two monoclonal antibodies reacting with surface structures on exclusively parathyroid cells and proximal kidney tubule cells were evaluated in the microscopic diagnosis of parathyroid disease. Immunostained cryosections of adenomatous and hyperplastic parathyroid glands from 30 hypercalcemic patients with primary or uremic hyperparathroidism (HPT) showed a reduced and heterogeneous staining with the antibodies. This contrasted with the intense and even staining of normal and suppressed human parathyroid glands, as well as lymph node metastases of parathyroid carcinoma. Fine-needle aspiration biopsies and imprints of the parathyroid glands showed a conspicuous antibody staining that enabled the identification of parathyroid cells. The antibodies seem to provide new means for histologic and cytologic identification of normal and pathologic parathyroid tissue, in conjunction with their highly restricted tissue reactivity. Because one of the antibodies interacts with a surface receptor mechanism of the parathyroid cells that is involved in the sensing and gating of calcium and, thereby, also with the regulation of parathyroid hormone release, the unequivocal demonstration of reduced staining of the adenomatous and hyperplastic parathyroid tissue suggests that HPT is associated with reduced expression of the calcium sensoring mechanism of the pathologic parathyroid tissue.
在甲状旁腺疾病的显微镜诊断中，对两种仅与甲状旁腺细胞和近端肾小管细胞表面结构发生反应的单克隆抗体进行了评估。对30例原发性或尿毒症性甲状旁腺功能亢进（HPT）高钙血症患者的腺瘤性和增生性甲状旁腺进行免疫染色的冰冻切片显示，这些抗体染色减弱且不均匀。这与正常和受抑制的人类甲状旁腺以及甲状旁腺癌的淋巴结转移灶的强烈且均匀的染色形成对比。甲状旁腺的细针穿刺活检和印片显示有明显的抗体染色，从而能够识别甲状旁腺细胞。这些抗体似乎为正常和病理性甲状旁腺组织的组织学和细胞学鉴定提供了新方法，同时它们具有高度受限的组织反应性。由于其中一种抗体与甲状旁腺细胞的一种表面受体机制相互作用，该机制参与钙的感知和门控，进而也与甲状旁腺激素释放的调节有关，腺瘤性和增生性甲状旁腺组织染色减弱的明确证明表明，HPT与病理性甲状旁腺组织钙感知机制的表达降低有关。
