Bjerneroth Gunnel, Juhlin Claes, Grimelius Lars, Rastad Jonas, Åkerström Göran
Department of Surgery, University Hospital, S-75185, Uppsala, Sweden.
Department Pathology, University Hospital, Uppsala, Sweden.
Endocr Pathol. 1992 Jun;3(2):83-92. doi: 10.1007/BF02921347.
The monoclonal antiparathyroid antibody E11 reacts with a glycoprotein of high molecular weight, which acts as a calcium receptor on the surface of parathyroid cells and mediates calcium regulation of parathyroid hormone (PTH) release. Reduced expression of the calcium receptor has been implicated as a cause of the defect in PTH regulation in the pathological parathyroid parenchyma of patients with hyperparathyroidism (HPT). The present study evaluated the efficacy of immunostainings with the E11 antibody in comparison with routine histopathological methods including staining by the oil red O technique for histological discrimination between normal and pathological parathyroid glands. Parathyroid tissue from euparathyroid individuals invariably presented intense and homogeneous surface staining, with the antibody on virtually all chief cells, while the pathological glands from patients with HPT consistently showed heterogeneous and reduced immunostaining. Even minimally enlarged pathological glands from individuals with mild hypercalcemia and the normal-sized glands associated with adenomas displayed parathyroid chief cells with reduced antibody reactivity. The monoclonal antiparathyroid antibody should constitute a useful tool in parathyroid histopathology not only by its ability to identify the parathyroid tissue, but also by directly demonstrating the functionally normal and abnormal cells within the parathyroid tissue.
单克隆抗甲状旁腺抗体E11与一种高分子量糖蛋白发生反应，该糖蛋白作为甲状旁腺细胞表面的钙受体，介导甲状旁腺激素（PTH）释放的钙调节。钙受体表达降低被认为是甲状旁腺功能亢进症（HPT）患者病理性甲状旁腺实质中PTH调节缺陷的一个原因。本研究评估了与常规组织病理学方法（包括油红O染色技术）相比，E11抗体免疫染色在区分正常和病理性甲状旁腺组织学方面的效果。来自甲状旁腺功能正常个体的甲状旁腺组织总是呈现强烈且均匀的表面染色，几乎所有主细胞上都有抗体，而HPT患者的病理性腺体始终显示出异质性且免疫染色减少。即使是来自轻度高钙血症个体的最小程度增大的病理性腺体以及与腺瘤相关的正常大小腺体，其甲状旁腺主细胞的抗体反应性也降低。单克隆抗甲状旁腺抗体不仅因其识别甲状旁腺组织的能力，还因其能直接显示甲状旁腺组织内功能正常和异常的细胞，应成为甲状旁腺组织病理学中的一种有用工具。
