Hao Lanxiang, Zhang Jing, Zhang Yonghong, Hu Haitao, Shao Weiwei, Zhang Xiaochen, Geng Chunmei, Wang Yanyan, Jiang Ling
Department of Endocrinology, Qilu Hospital, Shandong UniversityJinan 250012, China; Yancheng City No. 1 People's HospitalYancheng 224001, China.
Department of Endocrinology, Qilu Hospital, Shandong University Jinan 250012, China.
Am J Transl Res. 2016 Oct 15;8(10):4195-4204. eCollection 2016.
To investigate the effects of Bisphenol A (BPA) on prolactin (PRL) release, pituitary cell proliferation, prolactinoma formation in estrogen-sensitive Fischer 344 (F344) rats.
Four-week-old female F344 rats were orally administered with different concentrations of BPA or intraperitoneal injection of estradiol benzoate (estradiolbenzoate, E2) for 12 weeks. Bodyweight, blood RPL level and pituitary weights were observed and recorded. Real-time PCR, western blot and immunohistochemistry analysis were used to detect the mRNA and protein levels of the proliferation markers, including proliferating cell neclear antigen (PCNA), pituitary tumor-transforming gene (PTTG) and its relevant marker ERα. Plasma and urine BPA concentration in patients with prolactinoma and healthy participants were measured as well.
Body weights of the rats treated with BPA were significantly decreased compared with those in the control group. The plasma PRL level and the pituitary weights of the rats were higher than those in the control group after BPA treatment. Compared with the control group, the pituitary mRNA and protein expression levels of PCNA and PTTG were significantly increased after BPA treatment. Moreover, ERα expression level was enhanced by the treatment of BPA in comparison with that of the control group. Finally, the plasma BPA concentration in the prolactin tumor patients was significantly higher than that in the healthy participants.
BPA can significantly promote pituitary cell proliferation and prolactin secretion in F344 rats, which may have impact on the proliferation and secretion of pituitary cell function through the ERα pathway.
研究双酚A（BPA）对雌激素敏感的Fischer 344（F344）大鼠催乳素（PRL）释放、垂体细胞增殖及催乳素瘤形成的影响。
对4周龄雌性F344大鼠口服不同浓度的BPA或腹腔注射苯甲酸雌二醇（estradiolbenzoate，E2），持续12周。观察并记录体重、血RPL水平和垂体重量。采用实时荧光定量PCR、蛋白质免疫印迹法和免疫组织化学分析检测增殖标志物的mRNA和蛋白质水平，包括增殖细胞核抗原（PCNA）、垂体肿瘤转化基因（PTTG）及其相关标志物ERα。同时测定催乳素瘤患者和健康参与者血浆及尿液中的BPA浓度。
与对照组相比，BPA处理组大鼠体重显著降低。BPA处理后，大鼠血浆PRL水平和垂体重量高于对照组。与对照组相比，BPA处理后垂体PCNA和PTTG的mRNA及蛋白质表达水平显著升高。此外，与对照组相比，BPA处理可增强ERα表达水平。最后，催乳素瘤患者血浆BPA浓度显著高于健康参与者。
BPA可显著促进F344大鼠垂体细胞增殖和催乳素分泌，可能通过ERα途径影响垂体细胞功能的增殖和分泌。
