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丝裂原刺激后T细胞表面抗原表达缺陷。可控性小鼠损伤后淋巴细胞功能障碍的一个指标。

Defective T-cell surface antigen expression after mitogen stimulation. An index of lymphocyte dysfunction after controlled murine injury.

作者信息

Gadd M A, Hansbrough J F, Hoyt D B, Ozkan N

机构信息

Department of Surgery, University of California, San Diego.

出版信息

Ann Surg. 1989 Jan;209(1):112-8. doi: 10.1097/00000658-198901000-00016.

DOI:10.1097/00000658-198901000-00016

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1493887/

Abstract

Murine spleen T-cell activation in lectin-stimulated cultures after 25% body surface area burn injury or hind-limb amputation was studied by measuring the temporal expression of cell surface markers using monoclonal antibodies and two-color flow cytometry. Lymphocyte activation has been shown to be accompanied by the appearance of new surface antigens, including Interleukin-2 (IL-2) deceptor (IL-2R) and Ia, and emergence of cells that coexpress helper (Th) and suppressor (Ts) surface markers. IL-2R has been shown to appear early on stimulated cells, before DNA synthesis, whereas Ia appears later. Surface markers (L3T4, Lyt2, Ia, and IL-2R) were analyzed at time 0 and after 24, 48, and 72 hours of mitogen-stimulated culture. The appearance of IL-2R and Ia on Th (L3T4+) and Ts (Lyt-2+) populations was markedly depressed after burn injury, but minimal changes were seen after musculoskeletal injury. In addition, coexpression of L3T4/Lyt2 antigens was markedly reduced in burn-derived cells. Serum from burn-injured animals caused depression of surface antigen expression by stimulated normal cells. Recombinant IL-2, when added to burn-derived cell cultures, did not increase expression of these surface markers during culture, nor did it improve proliferation.

摘要

通过使用单克隆抗体和双色流式细胞术测量细胞表面标志物的时间表达，研究了25%体表面积烧伤或后肢截肢后凝集素刺激培养物中小鼠脾脏T细胞的活化情况。淋巴细胞活化已被证明伴随着新表面抗原的出现，包括白细胞介素-2（IL-2）受体（IL-2R）和Ia，以及共表达辅助（Th）和抑制（Ts）表面标志物的细胞的出现。IL-2R已被证明在刺激细胞上DNA合成之前就早期出现，而Ia出现较晚。在有丝分裂原刺激培养的0小时、24小时、48小时和72小时后分析表面标志物（L3T4、Lyt2、Ia和IL-2R）。烧伤后Th（L3T4+）和Ts（Lyt-2+）群体上IL-2R和Ia的出现明显受到抑制，但肌肉骨骼损伤后变化最小。此外，烧伤来源细胞中L3T4/Lyt2抗原的共表达明显降低。烧伤动物的血清导致刺激的正常细胞表面抗原表达降低。将重组IL-2添加到烧伤来源的细胞培养物中，在培养过程中不会增加这些表面标志物的表达，也不会改善增殖。