Nguyen Linda, Bohlen Joseph, Stricker Janelle, Chahal Ikttesh, Zhang Hanting, Pistilli Emidio E
Departments of Behavioral Medicine & Psychiatry and Physiology & Pharmacology, West Virginia University School of Medicine, Morgantown, WV, 26506, USA.
Division of Exercise Physiology, West Virginia University School of Medicine, Morgantown, WV, 26506, USA.
Metab Brain Dis. 2017 Apr;32(2):297-302. doi: 10.1007/s11011-016-9930-y. Epub 2016 Nov 11.
A hippocampus-specific IL15RαKO mouse (hipIl15ra /Cre) was generated to test the hypothesis that the targeted deletion of interleukin-15 receptor alpha (IL-15Rα) in the hippocampus contributes to altered behavior, including greater levels of anxiety and ambulatory activity. Using Cre-loxP, exons 2 and 3 of the IL-15Rα gene were excised within the hippocampus, while normal expression was maintained within the rest of the brain. In the open field test (OFT), hipIl15ra /Cre spent a greater amount of time in the periphery and less time in the central portions of the chamber, and there was also a noticeable trend for decreased rearing activity; these behaviors are consistent with greater levels of anxiety-like behavior in these mice. However, there were no differences in the overall locomotor counts in the OFT when comparing hipIl15ra /Cre mice to their littermate controls. These data implicate IL-15-related signaling within the hippocampus has a role in anxiety-like behavior.
为了验证海马体中白细胞介素-15受体α（IL-15Rα）的靶向缺失会导致行为改变（包括更高水平的焦虑和活动能力）这一假设，我们构建了一种海马体特异性IL15Rα基因敲除小鼠（hipIl15ra /Cre）。利用Cre-loxP系统，在海马体内切除了IL-15Rα基因的第2和第3外显子，而在大脑的其他部分维持正常表达。在旷场试验（OFT）中，hipIl15ra /Cre小鼠在试验箱外周花费的时间更多，在中央部分花费的时间更少，并且竖毛活动减少的趋势也很明显；这些行为与这些小鼠更高水平的焦虑样行为一致。然而，将hipIl15ra /Cre小鼠与其同窝对照小鼠相比，旷场试验中的总体运动计数没有差异。这些数据表明，海马体内与IL-15相关的信号传导在焦虑样行为中起作用。
