Zhao Qi, Yang Yi, Wang Changlin, Hou Ying, Chen Hui
Department of Pediatric Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Mol Med Rep. 2016 Dec;14(6):5111-5115. doi: 10.3892/mmr.2016.5914. Epub 2016 Nov 1.
Congenital obstructive nephropathy is the primary cause of chronic renal failure in children. Disorders of mitochondrial energy metabolism may be a primary factor underlying tubular cell apoptosis in hydronephrosis. The β-F1-ATPase (ATP5B) and electron transfer flavoprotein β subunit (ETFB) metabolic markers are involved in mitochondrial energy metabolism in other diseases. The aim of the present study was to evaluate whether ATP5B and ETFB are represented in the hydronephrotic kidney, and whether they are associated with the progression of hydronephrosis. The cohort examined consisted of 20 children with hydronephrosis, graded III and IV using the Society for Fetal Urology grading system, and a control group consisting of 20 patients with nephroblastoma. Reverse transcription‑quantitative polymerase chain reaction and immunoblot analyses were used to investigate the differential expression of genes and proteins in the two groups. The gene and protein expression levels of ATP5B and ETFB were upregulated in the hydronephrosis group. Correlation analyses revealed negative correlations between ATP5B, ETFB protein and split renal function (SRF). Receiver‑operator curve analysis found a diagnostic profile of the ETFB protein in identifying children with hydronephrosis with abnormal SRF (<45%). These results suggested that increasing levels of ATP5B and ETFB were associated with worsening renal injury. ATP5B and ETFB may be novel markers in hydronephrosis and require further detailed investigation.
先天性梗阻性肾病是儿童慢性肾衰竭的主要原因。线粒体能量代谢紊乱可能是肾积水时肾小管细胞凋亡的主要因素。β-F1-ATP酶（ATP5B）和电子传递黄素蛋白β亚基（ETFB）代谢标志物参与其他疾病的线粒体能量代谢。本研究的目的是评估ATP5B和ETFB在肾积水肾脏中的表达情况，以及它们是否与肾积水的进展相关。所检查的队列包括20例使用胎儿泌尿外科学会分级系统分级为III级和IV级的肾积水患儿，以及一个由20例肾母细胞瘤患者组成的对照组。采用逆转录-定量聚合酶链反应和免疫印迹分析来研究两组中基因和蛋白质的差异表达。肾积水组中ATP5B和ETFB的基因和蛋白质表达水平上调。相关性分析显示ATP5B、ETFB蛋白与分肾功能（SRF）之间呈负相关。受试者工作特征曲线分析发现ETFB蛋白在识别SRF异常（<45%）的肾积水患儿方面具有诊断特征。这些结果表明，ATP5B和ETFB水平升高与肾损伤加重有关。ATP5B和ETFB可能是肾积水的新型标志物，需要进一步详细研究。
