Silberstein D S, Schoof D D, Rodrick M L, Tai P C, Spry C J, David J R, Eberlein T J
Department of Medicine, Harvard Medical School, Boston, MA.
J Immunol. 1989 Mar 15;142(6):2162-7.
Of 16 patients, a total of 13 who received IL-2 and autologous IL-2-generated lymphokine-activated killer LAK cells developed eosinophilia late during the course of treatment. To understand the direct or indirect effects of IL-2 on eosinophils, the physical and functional characteristics of the late-treatment eosinophils were compared to those of early-treatment and control eosinophils. Late-treatment eosinophils differed from early-treatment and control eosinophils in the following respects: they had somewhat reduced density, hypersegmented nuclei, eosinophil cationic protein converted from the storage form to the secretory form, and a greater than 200% increased ability to kill larvae of Schistosoma mansoni by an antibody-dependent mechanism (cytotoxic function). In vitro, IL-2 (1000 U/ml in medium as used to culture LAK cells) did not affect the cytotoxic function of eosinophils from cancer patients or from control subjects. However, LAK cell-conditioned medium enhanced the cytotoxic function of eosinophils from early-treatment cancer patients and from normal subjects by greater than 150%. Thus, eosinophils late in the course of IL-2/LAK cell treatment undergo physical changes and become functionally activated. The involvement of IL-2 in these changes is probably indirect, as an inducer of factors that enhance eosinophil function.
在16例患者中，共有13例接受白细胞介素-2（IL-2）和自体IL-2产生的淋巴因子激活的杀伤细胞（LAK细胞）治疗的患者在治疗过程后期出现嗜酸性粒细胞增多。为了解IL-2对嗜酸性粒细胞的直接或间接影响，将治疗后期嗜酸性粒细胞的物理和功能特性与治疗早期和对照嗜酸性粒细胞进行了比较。治疗后期嗜酸性粒细胞在以下方面与治疗早期和对照嗜酸性粒细胞不同：它们的密度有所降低，细胞核分叶过多，嗜酸性粒细胞阳离子蛋白从储存形式转变为分泌形式，并且通过抗体依赖性机制（细胞毒性功能）杀死曼氏血吸虫幼虫的能力增加了200%以上。在体外，IL-2（用于培养LAK细胞的培养基中为1000 U/ml）不影响癌症患者或对照受试者嗜酸性粒细胞的细胞毒性功能。然而，LAK细胞条件培养基使治疗早期癌症患者和正常受试者的嗜酸性粒细胞的细胞毒性功能增强了150%以上。因此，在IL-2/LAK细胞治疗过程后期的嗜酸性粒细胞会发生物理变化并在功能上被激活。IL-2参与这些变化可能是间接的，作为增强嗜酸性粒细胞功能的因子的诱导剂。
