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盐胁迫下质膜H-ATP酶的上调可能使其能够应对胁迫。

Up-regulation of plasma membrane H-ATPase under salt stress may enable to cope with stress.

作者信息

Olfatmiri Hosna, Alemzadeh Abbas, Zakipour Zahra

机构信息

Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Iran.

出版信息

Mol Biol Res Commun. 2014 Mar;3(1):67-75.

Abstract

Most plants encounter stress such as drought and salinity that adversely affect growth, development and crop productivity. The expression of the gene glutathione-s-transferases (GST) extends throughout various protective mechanisms in plants and allows them to adapt to unfavorable environmental conditions. (the first phi GSTFs class) gene expression patterns in the wheat cultivars Mahuti and Alamut were studied under salt and ABA treatments using a qRT-PCR technique. Results showed that gene expression patterns were significantly different in these two cultivars. Data showed that in Mahuti, there was an increase of transcript accumulation under salt and ABA treatments at 3h, 10h and 72h respectively. In Alamut, however, the pattern of transcript accumulation was different; the maximum was at 3h. In contrast, there were no significant differences observed between the cultivars for gene expression profiles at three levels of NaCl concentration (50, 100, and 200 mM) or in ABA (Abscisic Acid) treatment. It is likely that difference of gene expression patterns between the cultivars (Mahuti as a salt tolerant cultivar and Alamut as a salt sensitive cultivar) is due to distinct signaling pathways which activate expression. Lack of a significant difference between the gene expression profile under salt and ABA treatments suggests that the gene is not induced by stress stimuli. Of course it is possible that other levels of NaCl and ABA treatments cause a change in the gene.

摘要

大多数植物会遭遇干旱和盐碱化等胁迫,这些胁迫会对植物的生长、发育及作物产量产生不利影响。谷胱甘肽 -S-转移酶(GST)基因的表达贯穿于植物的各种保护机制中,使植物能够适应不利的环境条件。利用实时荧光定量聚合酶链反应(qRT-PCR)技术,研究了小麦品种Mahuti和Alamut在盐和脱落酸(ABA)处理下(第一类phi GSTFs基因)的基因表达模式。结果表明,这两个品种的基因表达模式存在显著差异。数据显示,在Mahuti中,盐和ABA处理分别在3小时、10小时和72小时时转录本积累增加。然而,在Alamut中,转录本积累模式不同,最大值出现在3小时。相比之下,在三种氯化钠浓度(50、100和200 mM)或ABA(脱落酸)处理下,两个品种的基因表达谱没有显著差异。两个品种(Mahuti为耐盐品种,Alamut为盐敏感品种)之间基因表达模式的差异可能是由于激活表达的信号通路不同。盐和ABA处理下基因表达谱缺乏显著差异表明该基因不受胁迫刺激诱导。当然,其他水平的氯化钠和ABA处理可能会导致该基因发生变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d517/5019221/3d6320deb903/mbrc-3-067-g001.jpg

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