Diao Xiaolin, Wang Juan, Zhu Hong, He Bei
Department of Respiratory Medicine, Peking University Third Hospital, No. 49 Huayuan North Road, Haidian District, Beijing, 100191, China.
BMC Pulm Med. 2016 Nov 15;16(1):149. doi: 10.1186/s12890-016-0317-y.
Programmed cell death 5 (PDCD5) was first identified as an apoptosis-promoting protein and involved in some autoimmune diseases and inflammatory processes. Our previous study demonstrated greater expression of serum PDCD5 in asthmatic patients than controls. This study aimed to further explore the significance of PDCD5 in mice with induced allergic asthma.
We divided 16 female mice into 2 groups: control (n = 8) and allergen (ovalbumin, OVA)-challenged mice (n = 8). The modified ovalbumin inhalation method was used to generate the allergic asthma mouse model, and the impact of OVA was assessed by histology of lung tissue and morphometry. The number of cells in bronchoalveolar lavage fluid (BALF) was detected. Pulmonary function was measured by pressure sensors. PDCD5 and active caspase-3 levels were detected.
The expression of PDCD5 was higher with OVA challenge than for controls (p < 0.05). PDCD5 level was correlated with number of inflammatory cells in BALF and lung function. Moreover, active caspase-3 level was increased in the OVA-challenged mice (p < 0.001) and correlated with PDCD5 level (p = 0.000).
These data demonstrate an association between level of PDCD5 and asthma severity and indicate that PDCD5 may play a role in allergic asthma.
程序性细胞死亡5（PDCD5）最初被鉴定为一种促凋亡蛋白，并参与一些自身免疫性疾病和炎症过程。我们之前的研究表明，哮喘患者血清中PDCD5的表达高于对照组。本研究旨在进一步探讨PDCD5在诱导性变应性哮喘小鼠中的意义。
我们将16只雌性小鼠分为2组：对照组（n = 8）和变应原（卵清蛋白，OVA）激发组（n = 8）。采用改良的卵清蛋白吸入法建立变应性哮喘小鼠模型，并通过肺组织组织学和形态计量学评估OVA的影响。检测支气管肺泡灌洗液（BALF）中的细胞数量。用压力传感器测量肺功能。检测PDCD5和活性半胱天冬酶-3水平。
OVA激发组中PDCD5的表达高于对照组（p < 0.05）。PDCD5水平与BALF中的炎症细胞数量和肺功能相关。此外，OVA激发组小鼠的活性半胱天冬酶-3水平升高（p < 0.001），且与PDCD5水平相关（p = 0.000）。
这些数据表明PDCD5水平与哮喘严重程度之间存在关联，并表明PDCD5可能在变应性哮喘中起作用。
