Chen Yingyu, Zou Zhiqiang, Xu Ailing, Liu Youde, Pan Huan, Jin Lei
Key Laboratory of Medical Immunology, Ministry of Health, Peking University, Beijing, China.
J Immunoassay Immunochem. 2013;34(3):294-304. doi: 10.1080/15321819.2012.731461.
Intracellular protein molecules are detected in the blood following release from damaged cells. PDCD5 is widely expressed in most types of normal human tissue and is unregulated in cells undergoing apoptosis. It is therefore hypothesized that release of PDCD5 into the circulation might be a specific marker of apoptosis. In this study, a sandwich ELISA was developed for quantification of soluble PDCD5 protein and used to investigate serum PDCD5 levels in liver diseases. The highest levels of PDCD5 were detected in acute icteric hepatitis (AIH) patients compared with normal subjects and other detected liver diseases, such as chronic active hepatitis B (CAHB), chronic persistent hepatitis B (CPHB) and and liver cirrhosis (LC). Increased PDCD5 levels correlated well with ALT and AST in AIH and CAHB patients. In patients with CPHB, increased PDCD5 levels correlated well with AST, TBI, DBIL, and IBIL. In LC patients, PDCD5 levels correlated well with AST/ALT and DBIL. More importantly, increased PDCD5 levels were also observed in patients with normal ALT or AST levels. These data demonstrate a correlation between increased levels of PDCD5 in serum and liver disease progression and indicate the potential utility of serum PDCD5 as a biomarker for monitoring liver injury.
细胞内蛋白质分子在从受损细胞释放后可在血液中被检测到。PDCD5在大多数类型的正常人体组织中广泛表达，并且在经历凋亡的细胞中表达上调。因此，有人推测PDCD5释放到循环系统中可能是凋亡的一个特异性标志物。在本研究中，开发了一种夹心ELISA法用于定量可溶性PDCD5蛋白，并用于研究肝脏疾病患者血清中PDCD5的水平。与正常受试者以及其他所检测的肝脏疾病（如慢性乙型活动性肝炎（CAHB）、慢性乙型迁延性肝炎（CPHB）和肝硬化（LC））相比，急性黄疸型肝炎（AIH）患者的PDCD5水平最高。在AIH和CAHB患者中，PDCD5水平升高与谷丙转氨酶（ALT）和谷草转氨酶（AST）密切相关。在CPHB患者中，PDCD5水平升高与AST、总胆红素（TBI）、直接胆红素（DBIL）和间接胆红素（IBIL）密切相关。在LC患者中，PDCD5水平与AST/ALT和DBIL密切相关。更重要的是，在ALT或AST水平正常的患者中也观察到PDCD5水平升高。这些数据表明血清中PDCD5水平升高与肝脏疾病进展之间存在相关性，并表明血清PDCD5作为监测肝损伤生物标志物的潜在用途。
