评估苏氨酰-tRNA合成酶对血管生成相关反应的影响。
Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses.
作者信息
Mirando Adam C, Abdi Khadar, Wo Peibin, Lounsbury Karen M
机构信息
Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.
Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.
出版信息
Methods. 2017 Jan 15;113:132-138. doi: 10.1016/j.ymeth.2016.11.007. Epub 2016 Nov 12.
Several recent reports have found a connection between specific aminoacyl-tRNA synthetases and the regulation of angiogenesis. As this new area of research is explored, it is important to have reliable assays to assess the specific angiogenesis functions of these enzymes. This review provides information about specific in vitro and in vivo methods that were used to assess the angiogenic functions of threonyl-tRNA synthetase including endothelial cell migration and tube assays as well as chorioallantoic membrane and tumor vascularization assays. The theory and discussion include best methods of analysis and quantification along with the advantages and limitations of each type of assay.
最近的几份报告发现特定的氨酰-tRNA合成酶与血管生成的调节之间存在联系。随着这一研究新领域的不断探索，拥有可靠的检测方法来评估这些酶的特定血管生成功能非常重要。本综述提供了有关用于评估苏氨酰-tRNA合成酶血管生成功能的特定体外和体内方法的信息，包括内皮细胞迁移和管腔检测以及绒毛尿囊膜和肿瘤血管生成检测。理论和讨论包括最佳分析和定量方法以及每种检测类型的优缺点。
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